Fluorescent amplified-fragment length polymorphism subtyping of the Salmonella enterica serovar Enteritidis phage type 4 clone complex

M. Desai, E. J. Threlfall, J. Stanley

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Fluorescent amplified-fragment length polymorphism (FAFLP) analysis, a high-resolution PCR-based genome fingerprinting method, was used to subtype Salmonella enterica serovar Enteritidis phage type 4. This single phage type is responsible for the majority of salmonellosis in Europe. Twenty strains isolated from nine outbreaks, five isolates from sporadic cases of human infection, four strains of poultry origin, and one laboratory-derived strain were comparatively studied by pulsed-field gel electrophoresis (PFGE) and FAFLP analysis. Following macrorestriction with XbaI, PFGE classified 73% of PT4 strains as a single type. FAFLP analysis was carried out with the primer pair EcoRI+0 and MseI+C, by simultaneously sampling 170 to 190 loci throughout the PT4 genome. Twenty-three FAFLP profiles, with 1 to 61 amplified-fragment differences, were found among the 30 strains. The index of discriminatory power of FAFLP analysis was 0.98, compared to 0.47 for PFGE. FAFLP analysis assigned genotypes to each PT4 outbreak, as well as sporadic PT4 infections, a significant development for the epidemiology and control of this zoonotic enteric pathogen.

Original languageEnglish (US)
Pages (from-to)201-206
Number of pages6
JournalJournal of clinical microbiology
Volume39
Issue number1
DOIs
StatePublished - Jan 30 2001

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Amplified Fragment Length Polymorphism Analysis
Salmonella enteritidis
Bacteriophages
Clone Cells
Pulsed Field Gel Electrophoresis
Disease Outbreaks
Genome
Salmonella Infections
Zoonoses
Poultry
Infection
Epidemiology
Genotype
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

Cite this

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abstract = "Fluorescent amplified-fragment length polymorphism (FAFLP) analysis, a high-resolution PCR-based genome fingerprinting method, was used to subtype Salmonella enterica serovar Enteritidis phage type 4. This single phage type is responsible for the majority of salmonellosis in Europe. Twenty strains isolated from nine outbreaks, five isolates from sporadic cases of human infection, four strains of poultry origin, and one laboratory-derived strain were comparatively studied by pulsed-field gel electrophoresis (PFGE) and FAFLP analysis. Following macrorestriction with XbaI, PFGE classified 73{\%} of PT4 strains as a single type. FAFLP analysis was carried out with the primer pair EcoRI+0 and MseI+C, by simultaneously sampling 170 to 190 loci throughout the PT4 genome. Twenty-three FAFLP profiles, with 1 to 61 amplified-fragment differences, were found among the 30 strains. The index of discriminatory power of FAFLP analysis was 0.98, compared to 0.47 for PFGE. FAFLP analysis assigned genotypes to each PT4 outbreak, as well as sporadic PT4 infections, a significant development for the epidemiology and control of this zoonotic enteric pathogen.",
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Fluorescent amplified-fragment length polymorphism subtyping of the Salmonella enterica serovar Enteritidis phage type 4 clone complex. / Desai, M.; Threlfall, E. J.; Stanley, J.

In: Journal of clinical microbiology, Vol. 39, No. 1, 30.01.2001, p. 201-206.

Research output: Contribution to journalArticle

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