Fluorometric assay of bethanidine in plasma

C. N. Corder, T. S. Klaniecki, R. H. Mc Donald, C. Berlin

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A fluorometric method for determining bethanidine in blood plasma is described. The bethanidine is extracted into chloroform, a drug‐dye complex with eosin Y is formed, and the fluorescence is measured (excitation, 535 nm; fluorescence, 560 nm). The assay detects 0.02 μM bethanidine (4 ng/ml) in plasma. The relative fluorescence of several body constituents and antihypertensive drugs is negligible. The plasma levels of bethanidine in four hypertensive patients receiving this drug were measured.

Original languageEnglish (US)
Pages (from-to)785-788
Number of pages4
JournalJournal of Pharmaceutical Sciences
Volume64
Issue number5
DOIs
StatePublished - May 1975

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Bethanidine
Fluorescence
Eosine Yellowish-(YS)
Chloroform
Antihypertensive Agents
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science

Cite this

Corder, C. N., Klaniecki, T. S., Mc Donald, R. H., & Berlin, C. (1975). Fluorometric assay of bethanidine in plasma. Journal of Pharmaceutical Sciences, 64(5), 785-788. https://doi.org/10.1002/jps.2600640511
Corder, C. N. ; Klaniecki, T. S. ; Mc Donald, R. H. ; Berlin, C. / Fluorometric assay of bethanidine in plasma. In: Journal of Pharmaceutical Sciences. 1975 ; Vol. 64, No. 5. pp. 785-788.
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Corder, CN, Klaniecki, TS, Mc Donald, RH & Berlin, C 1975, 'Fluorometric assay of bethanidine in plasma', Journal of Pharmaceutical Sciences, vol. 64, no. 5, pp. 785-788. https://doi.org/10.1002/jps.2600640511

Fluorometric assay of bethanidine in plasma. / Corder, C. N.; Klaniecki, T. S.; Mc Donald, R. H.; Berlin, C.

In: Journal of Pharmaceutical Sciences, Vol. 64, No. 5, 05.1975, p. 785-788.

Research output: Contribution to journalArticle

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Corder CN, Klaniecki TS, Mc Donald RH, Berlin C. Fluorometric assay of bethanidine in plasma. Journal of Pharmaceutical Sciences. 1975 May;64(5):785-788. https://doi.org/10.1002/jps.2600640511