Footprinting analysis demonstrates extensive similarity between eukaryotic RNase P and RNase MRP holoenzymes

Olga Esakova, Anna Perederina, Chao Quan, Mark E. Schmitt, Andrey S. Kiasilnikov

Research output: Contribution to journalArticle

23 Scopus citations


Eukaryotic ribonuclease (RNase) P and RNase MRP are evolutionary related RNA-based enzymes involved in metabolism of various RNA molecules, including tRNA and rRNA. In contrast to the closely related eubacterial RNase P, which is comprised of an RNA component and a single small protein, these enzymes contain multiple protein components. Here we report the results of footprinting studies performed on purified Saccharomyces cerevisiae RNase MRP and RNase P holoenzymes. The results identify regions of the RNA components affected by the protein moiety, suggest a role of the proteins in stabilization of the RNA fold, and point to substantial similarities between the two evolutionary related RNA-based enzymes.

Original languageEnglish (US)
Pages (from-to)1558-1567
Number of pages10
Issue number8
StatePublished - Aug 1 2008


All Science Journal Classification (ASJC) codes

  • Molecular Biology

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