Four products from Escherichia coli pseudogenes increase hydrogen production

Mohd Zulkhairi Mohd Yusoff, Yuya Hashiguchi, Toshinari Maeda, Thomas Keith Wood

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Pseudogenes are considered to be nonfunctional genes that lack a physiological role. By screening 3985 Escherichia coli mutants using chemochromic membranes, we found four pseudogenes involved in hydrogen metabolism. Knockouts of pseudogenes ydfW and ypdJ had a defective hydrogen phenotype on glucose and formate, respectively. Also, the knockout of pseudogene yqiG formed hydrogen from formate but not from glucose. For the yqiG mutant, 100% hydrogen recovery was obtained by the complementation of YqiG via a plasmid. The knockout of pseudogene ylcE showed hydrogen deficiency in minimal media which suggested that the role of YlcE is associated with cell growth. Hence, the products of these four pseudogenes play an important physiological role in hydrogen production in E. coli.

Original languageEnglish (US)
Pages (from-to)576-579
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume439
Issue number4
DOIs
StatePublished - Oct 4 2013

Fingerprint

Pseudogenes
Hydrogen production
formic acid
Escherichia coli
Hydrogen
Glucose
Cell growth
Metabolism
Screening
Plasmids
Genes
Membranes
Recovery
Phenotype
Growth

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Mohd Yusoff, Mohd Zulkhairi ; Hashiguchi, Yuya ; Maeda, Toshinari ; Wood, Thomas Keith. / Four products from Escherichia coli pseudogenes increase hydrogen production. In: Biochemical and Biophysical Research Communications. 2013 ; Vol. 439, No. 4. pp. 576-579.
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Four products from Escherichia coli pseudogenes increase hydrogen production. / Mohd Yusoff, Mohd Zulkhairi; Hashiguchi, Yuya; Maeda, Toshinari; Wood, Thomas Keith.

In: Biochemical and Biophysical Research Communications, Vol. 439, No. 4, 04.10.2013, p. 576-579.

Research output: Contribution to journalArticle

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