FOXA1 deletion in luminal epithelium causes prostatic hyperplasia and alteration of differentiated phenotype

David Degraff, Magdalena M. Grabowska, Tom C. Case, Xiuping Yu, Mary K. Herrick, William J. Hayward, Douglas W. Strand, Justin M. Cates, Simon W. Hayward, Nan Gao, Michael A. Walter, Ralph Buttyan, Yajun Yi, Klaus H. Kaestner, Robert J. Matusik

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The forkhead box (Fox) superfamily of transcription factors has essential roles in organogenesis and tissue differentiation. Foxa1 and Foxa2 are expressed during prostate budding and ductal morphogenesis, whereas Foxa1 expression is retained in adult prostate epithelium. Previous characterization of prostatic tissue rescued from embryonic Foxa1 knockout mice revealed Foxa1 to be essential for ductal morphogenesis and epithelial maturation. However, it is unknown whether Foxa1 is required to maintain the differentiated status in adult prostate epithelium. Here, we employed the PBCre4 transgenic system and determined the impact of prostate-specific Foxa1 deletion in adult murine epithelium. PBCre4/Foxa1loxp/loxp mouse prostates showed progressive florid hyperplasia with extensive cribriform patterning, with the anterior prostate being most affected. Immunohistochemistry studies show mosaic Foxa1 KO consistent with PBCre4 activity, with Foxa1 KO epithelial cells specifically exhibiting altered cell morphology, increased proliferation, and elevated expression of basal cell markers. Castration studies showed that, while PBCre4/Foxa1loxp/loxp prostates did not exhibit altered sensitivity in response to hormone ablation compared with control prostates, the number of Foxa1-positive cells in mosaic Foxa1 KO prostates was significantly reduced compared with Foxa1-negative cells following castration. Unexpectedly, gene expression profile analyses revealed that Foxa1 deletion caused abnormal expression of seminal vesicle-associated genes in KO prostates. In summary, these results indicate Foxa1 expression is required for the maintenance of prostatic cellular differentiation.

Original languageEnglish (US)
Pages (from-to)726-739
Number of pages14
JournalLaboratory Investigation
Volume94
Issue number7
DOIs
StatePublished - Jan 1 2014

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Prostatic Hyperplasia
Prostate
Epithelium
Phenotype
Castration
Morphogenesis
Forkhead Transcription Factors
Organogenesis
Seminal Vesicles
Transcriptome
Knockout Mice
Hyperplasia
Epithelial Cells
Immunohistochemistry
Maintenance
Hormones

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Cell Biology

Cite this

Degraff, David ; Grabowska, Magdalena M. ; Case, Tom C. ; Yu, Xiuping ; Herrick, Mary K. ; Hayward, William J. ; Strand, Douglas W. ; Cates, Justin M. ; Hayward, Simon W. ; Gao, Nan ; Walter, Michael A. ; Buttyan, Ralph ; Yi, Yajun ; Kaestner, Klaus H. ; Matusik, Robert J. / FOXA1 deletion in luminal epithelium causes prostatic hyperplasia and alteration of differentiated phenotype. In: Laboratory Investigation. 2014 ; Vol. 94, No. 7. pp. 726-739.
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abstract = "The forkhead box (Fox) superfamily of transcription factors has essential roles in organogenesis and tissue differentiation. Foxa1 and Foxa2 are expressed during prostate budding and ductal morphogenesis, whereas Foxa1 expression is retained in adult prostate epithelium. Previous characterization of prostatic tissue rescued from embryonic Foxa1 knockout mice revealed Foxa1 to be essential for ductal morphogenesis and epithelial maturation. However, it is unknown whether Foxa1 is required to maintain the differentiated status in adult prostate epithelium. Here, we employed the PBCre4 transgenic system and determined the impact of prostate-specific Foxa1 deletion in adult murine epithelium. PBCre4/Foxa1loxp/loxp mouse prostates showed progressive florid hyperplasia with extensive cribriform patterning, with the anterior prostate being most affected. Immunohistochemistry studies show mosaic Foxa1 KO consistent with PBCre4 activity, with Foxa1 KO epithelial cells specifically exhibiting altered cell morphology, increased proliferation, and elevated expression of basal cell markers. Castration studies showed that, while PBCre4/Foxa1loxp/loxp prostates did not exhibit altered sensitivity in response to hormone ablation compared with control prostates, the number of Foxa1-positive cells in mosaic Foxa1 KO prostates was significantly reduced compared with Foxa1-negative cells following castration. Unexpectedly, gene expression profile analyses revealed that Foxa1 deletion caused abnormal expression of seminal vesicle-associated genes in KO prostates. In summary, these results indicate Foxa1 expression is required for the maintenance of prostatic cellular differentiation.",
author = "David Degraff and Grabowska, {Magdalena M.} and Case, {Tom C.} and Xiuping Yu and Herrick, {Mary K.} and Hayward, {William J.} and Strand, {Douglas W.} and Cates, {Justin M.} and Hayward, {Simon W.} and Nan Gao and Walter, {Michael A.} and Ralph Buttyan and Yajun Yi and Kaestner, {Klaus H.} and Matusik, {Robert J.}",
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Degraff, D, Grabowska, MM, Case, TC, Yu, X, Herrick, MK, Hayward, WJ, Strand, DW, Cates, JM, Hayward, SW, Gao, N, Walter, MA, Buttyan, R, Yi, Y, Kaestner, KH & Matusik, RJ 2014, 'FOXA1 deletion in luminal epithelium causes prostatic hyperplasia and alteration of differentiated phenotype', Laboratory Investigation, vol. 94, no. 7, pp. 726-739. https://doi.org/10.1038/labinvest.2014.64

FOXA1 deletion in luminal epithelium causes prostatic hyperplasia and alteration of differentiated phenotype. / Degraff, David; Grabowska, Magdalena M.; Case, Tom C.; Yu, Xiuping; Herrick, Mary K.; Hayward, William J.; Strand, Douglas W.; Cates, Justin M.; Hayward, Simon W.; Gao, Nan; Walter, Michael A.; Buttyan, Ralph; Yi, Yajun; Kaestner, Klaus H.; Matusik, Robert J.

In: Laboratory Investigation, Vol. 94, No. 7, 01.01.2014, p. 726-739.

Research output: Contribution to journalArticle

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AU - Grabowska, Magdalena M.

AU - Case, Tom C.

AU - Yu, Xiuping

AU - Herrick, Mary K.

AU - Hayward, William J.

AU - Strand, Douglas W.

AU - Cates, Justin M.

AU - Hayward, Simon W.

AU - Gao, Nan

AU - Walter, Michael A.

AU - Buttyan, Ralph

AU - Yi, Yajun

AU - Kaestner, Klaus H.

AU - Matusik, Robert J.

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