Freeze-drying as sample preparation for micellar electrokinetic capillary chromatography-electrochemical separations of neurochemicals in drosophila brains

E. Carina Berglund, Nicholas J. Kuklinski, Ekin Karagündüz, Kubra Ucar, Jörg Hanrieder, Andrew G. Ewing

Research output: Contribution to journalArticlepeer-review

Abstract

Micellar electrokinetic capillary chromatography with electrochemical detection has been used to quantify biogenic amines in freeze-dried brains of Drosophila melanogaster. Freeze-drying samples offers a way to preserve the biological sample while making dissection of these tiny samples easier and faster. Fly samples were extracted in cold acetone and dried in a rotary evaporator. Extraction and drying times were optimized in order to avoid contamination by red pigment from the fly eyes and still have intact brain structures. Single freeze-dried fly brain samples were found to produce representative electropherograms as a single hand-dissected brain sample. With utilization of the faster dissection time that freeze-drying affords, the number of brains in a fixed homogenate volume can be increased to concentrate the sample. Thus, concentrated brain samples containing five or fifteen preserved brains were analyzed for their neurotransmitter content, and four analytes; N-acetyloctopamine, N-acetylserotonin, N-acetyltyramine, and N-acetyldopamine were found to correspond well with previously reported values.

Original languageEnglish (US)
Pages (from-to)2841-2846
Number of pages6
JournalAnalytical Chemistry
Volume85
Issue number5
DOIs
StatePublished - Mar 5 2013

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

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