Frequency division multiplexed fluorescence confocal microscopy

    Research output: Contribution to specialist publicationArticle

    1 Citation (Scopus)

    Abstract

    High-speed, frequency division multiplexed, multichannel fluorescence confocal microscopy imaging is achieved by encoding the spatial location information into the frequency domain. The technique provides high spatial resolution fluorescence imaging in real-time upto the nanosecond range when applied to the detection modules based on single or arrayed photomultiplier tubes. The crosstalk signal noise among various frequency channels is reduced by separating adjacent frequencies in the laser channels. For a Gaussian noise, the bandwidth of noise spectrum is estimated by observing the detected signal over a finite time interval. The maximum number of the frequency division multiplexed channels is limited by the response time of the fluorescence emission and the photomultiplier tube detector. The spatial resolution of the frequency division multiplexed fluorescence confocal microscope is same as conventional fluorescence confocal microscopes.

    Original languageEnglish (US)
    Pages38-42
    Number of pages5
    Volume13
    No12
    Specialist publicationBiophotonics International
    StatePublished - Dec 2006

    Fingerprint

    Confocal microscopy
    Fluorescence microscopy
    Fluorescence
    Photomultipliers
    Microscopes
    Gaussian noise (electronic)
    Imaging techniques
    Crosstalk
    Detectors
    Bandwidth
    Lasers

    All Science Journal Classification (ASJC) codes

    • Control and Systems Engineering

    Cite this

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    abstract = "High-speed, frequency division multiplexed, multichannel fluorescence confocal microscopy imaging is achieved by encoding the spatial location information into the frequency domain. The technique provides high spatial resolution fluorescence imaging in real-time upto the nanosecond range when applied to the detection modules based on single or arrayed photomultiplier tubes. The crosstalk signal noise among various frequency channels is reduced by separating adjacent frequencies in the laser channels. For a Gaussian noise, the bandwidth of noise spectrum is estimated by observing the detected signal over a finite time interval. The maximum number of the frequency division multiplexed channels is limited by the response time of the fluorescence emission and the photomultiplier tube detector. The spatial resolution of the frequency division multiplexed fluorescence confocal microscope is same as conventional fluorescence confocal microscopes.",
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    Frequency division multiplexed fluorescence confocal microscopy. / Yin, Shizhuo.

    In: Biophotonics International, Vol. 13, No. 12, 12.2006, p. 38-42.

    Research output: Contribution to specialist publicationArticle

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