B10.W females were immunized against syngeneic male cells (via the footpad and also i.p. in some strains) and their spleen cells were then restimulated in vitro and tested in the cell-mediated lympholysis assay for H-Y-specific killing of target cells. Only seven of the 33 tested lines were anti-H-Y responders. The effector cells obtained from each of the responder lines were then tested against male and female cells of other B10.W lines, as well as a number of classic B10 congenic lines, and the MHC molecules providing the context for H-Y recognition were identified. Theey were: K(k), K(w3), K(w17), K(w27), D(k), and D(p). None of the strains generated effector cells capable of recognizing the H-Y antigen simultaneously in the context of the K and D molecules. The WOA1 females generated effector cells by using the K(w7) molecule for context of recognition, whereas the WR7 females produced cells recognizing the H-Y antigen exclusively in the context of the D(k) molecule despite the fact that both lines share the K(w7) gene. Some of the effector cells cross-reacted with both male and female cells of other strains and this cross-reactivity could be attributed to the recognition of allogeneic MHC molecules controlled by K or D region genes. Interestingly, STA39 females generated D(p)- but not K(w3)-restricted anti-H-Y responses, whereas SAA48 females generated K(w3)- but not D(w3)-restricted responses; the K(w3)- restricted cells cross-reacted with the D(p) molecule. This cross-reaction might explain why the STA39 females do not mount a K(w3)-restricted anti-H-Y response. Because the K(w3) + H-Y combination resembles D(p), the anti-K(w3) + H-Y T cells are functionally eliminated when tolerance of D(p) molecules is attained in the STA39 mice.
|Original language||English (US)|
|Number of pages||4|
|Journal||Journal of Immunology|
|Publication status||Published - Jan 1 1983|
All Science Journal Classification (ASJC) codes
- Immunology and Allergy