Abstract

Dynamic analysis of oxygen (O2) has been limited by the lack of a real-time, quantitative, and biocompatible sensor. To address these demands, we designed a ratiometric optode matrix consisting of the phosphorescence quenching dye platinum (II) octaethylporphine ketone (PtOEPK) and nanocystal quantum dots (NQDs), which when embedded within an inert polymer matrix allows long-term pre-designed excitation through fluorescence resonance energy transfer (FRET). Depositing this matrix on various glass substrates allowed the development of a series of optical sensors able to measure interstitial oxygen concentration [O2] with several hundred millisecond temporal resolution in varying biological microdomains of active brain tissue.

Original languageEnglish (US)
Pages (from-to)45-51
Number of pages7
JournalJournal of Neuroscience Methods
Volume214
Issue number1
DOIs
StatePublished - Mar 2013

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

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