Abstract

Dynamic analysis of oxygen (O2) has been limited by the lack of a real-time, quantitative, and biocompatible sensor. To address these demands, we designed a ratiometric optode matrix consisting of the phosphorescence quenching dye platinum (II) octaethylporphine ketone (PtOEPK) and nanocystal quantum dots (NQDs), which when embedded within an inert polymer matrix allows long-term pre-designed excitation through fluorescence resonance energy transfer (FRET). Depositing this matrix on various glass substrates allowed the development of a series of optical sensors able to measure interstitial oxygen concentration [O2] with several hundred millisecond temporal resolution in varying biological microdomains of active brain tissue.

Original languageEnglish (US)
Pages (from-to)45-51
Number of pages7
JournalJournal of Neuroscience Methods
Volume214
Issue number1
DOIs
StatePublished - Mar 1 2013

Fingerprint

Fluorescence Resonance Energy Transfer
Oxygen
Quantum Dots
Glass
Polymers
Coloring Agents
Brain
platinum(II) octaethylporphine ketone

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Ingram, Justin M. ; Zhang, Chunfeng ; Xu, Jian ; Schiff, Steven. / FRET excited ratiometric oxygen sensing in living tissue. In: Journal of Neuroscience Methods. 2013 ; Vol. 214, No. 1. pp. 45-51.
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FRET excited ratiometric oxygen sensing in living tissue. / Ingram, Justin M.; Zhang, Chunfeng; Xu, Jian; Schiff, Steven.

In: Journal of Neuroscience Methods, Vol. 214, No. 1, 01.03.2013, p. 45-51.

Research output: Contribution to journalArticle

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