Function of the small hydrophobic protein of J paramyxovirus

Zhuo Li, Jie Xu, Jui Patel, Sandra Fuentes, Yuan Lin, Danielle Anderson, Kaori Sakamoto, Lin Fa Wang, Biao He

Research output: Contribution to journalArticle

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Abstract

At 18,954 nucleotides, the J paramyxovirus (JPV) genome is one of the largest in the family Paramyxoviridae, consisting of eight genes in the order 3′-N-P/V/C-M-F-SH-TM-G-L-5′. To study the function of novel paramyxovirus genes in JPV, a plasmid containing a full-length cDNA clone of the genome of JPV was constructed. In this study, the function of the small hydrophobic (SH) protein of JPV was examined by generating a recombinant JPV lacking the coding sequence of the SH protein (rJPVΔSH). rJPVΔSH was viable and had no growth defect in tissue culture cells. However, more tumor necrosis factor alpha (TNF-α) was produced during rJPVΔSH infection, suggesting that SH plays a role in inhibiting TNF-α production. rJPVΔSH induced more apoptosis in tissue culture cells than rJPV. Virus-induced apoptosis was inhibited by neutralizing antibody against TNF-α, suggesting that TNF-α contributes to JPV-induced apoptosis in vitro. The expression of JPV SH protein inhibited TNF-α-induced NF-κB activation in a reporter gene assay, suggesting that JPV SH protein can inhibit TNF-α signaling in vitro. Furthermore, infection of mice with rJPVΔSH induced more TNF-α expression, indicating that SH plays a role in blocking TNF-α expression in vivo.

Original languageEnglish (US)
Pages (from-to)32-42
Number of pages11
JournalJournal of virology
Volume85
Issue number1
DOIs
StatePublished - Jan 1 2011

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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    Li, Z., Xu, J., Patel, J., Fuentes, S., Lin, Y., Anderson, D., Sakamoto, K., Wang, L. F., & He, B. (2011). Function of the small hydrophobic protein of J paramyxovirus. Journal of virology, 85(1), 32-42. https://doi.org/10.1128/JVI.01673-10