Function of the small hydrophobic protein of J paramyxovirus

Zhuo Li, Jie Xu, Jui Patel, Sandra Fuentes, Yuan Lin, Danielle Anderson, Kaori Sakamoto, Lin Fa Wang, Biao He

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

At 18,954 nucleotides, the J paramyxovirus (JPV) genome is one of the largest in the family Paramyxoviridae, consisting of eight genes in the order 3′-N-P/V/C-M-F-SH-TM-G-L-5′. To study the function of novel paramyxovirus genes in JPV, a plasmid containing a full-length cDNA clone of the genome of JPV was constructed. In this study, the function of the small hydrophobic (SH) protein of JPV was examined by generating a recombinant JPV lacking the coding sequence of the SH protein (rJPVΔSH). rJPVΔSH was viable and had no growth defect in tissue culture cells. However, more tumor necrosis factor alpha (TNF-α) was produced during rJPVΔSH infection, suggesting that SH plays a role in inhibiting TNF-α production. rJPVΔSH induced more apoptosis in tissue culture cells than rJPV. Virus-induced apoptosis was inhibited by neutralizing antibody against TNF-α, suggesting that TNF-α contributes to JPV-induced apoptosis in vitro. The expression of JPV SH protein inhibited TNF-α-induced NF-κB activation in a reporter gene assay, suggesting that JPV SH protein can inhibit TNF-α signaling in vitro. Furthermore, infection of mice with rJPVΔSH induced more TNF-α expression, indicating that SH plays a role in blocking TNF-α expression in vivo.

Original languageEnglish (US)
Pages (from-to)32-42
Number of pages11
JournalJournal of virology
Volume85
Issue number1
DOIs
StatePublished - Jan 1 2011

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Respirovirus
tumor necrosis factor-alpha
Tumor Necrosis Factor-alpha
Proteins
proteins
Apoptosis
apoptosis
Paramyxoviridae
tissue culture
Cell Culture Techniques
Genome
Gene Order
genome
Infection
Neutralizing Antibodies
Reporter Genes
neutralizing antibodies
reporter genes
infection
Plasmids

All Science Journal Classification (ASJC) codes

  • Immunology
  • Virology

Cite this

Li, Z., Xu, J., Patel, J., Fuentes, S., Lin, Y., Anderson, D., ... He, B. (2011). Function of the small hydrophobic protein of J paramyxovirus. Journal of virology, 85(1), 32-42. https://doi.org/10.1128/JVI.01673-10
Li, Zhuo ; Xu, Jie ; Patel, Jui ; Fuentes, Sandra ; Lin, Yuan ; Anderson, Danielle ; Sakamoto, Kaori ; Wang, Lin Fa ; He, Biao. / Function of the small hydrophobic protein of J paramyxovirus. In: Journal of virology. 2011 ; Vol. 85, No. 1. pp. 32-42.
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Li, Z, Xu, J, Patel, J, Fuentes, S, Lin, Y, Anderson, D, Sakamoto, K, Wang, LF & He, B 2011, 'Function of the small hydrophobic protein of J paramyxovirus', Journal of virology, vol. 85, no. 1, pp. 32-42. https://doi.org/10.1128/JVI.01673-10

Function of the small hydrophobic protein of J paramyxovirus. / Li, Zhuo; Xu, Jie; Patel, Jui; Fuentes, Sandra; Lin, Yuan; Anderson, Danielle; Sakamoto, Kaori; Wang, Lin Fa; He, Biao.

In: Journal of virology, Vol. 85, No. 1, 01.01.2011, p. 32-42.

Research output: Contribution to journalArticle

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Li Z, Xu J, Patel J, Fuentes S, Lin Y, Anderson D et al. Function of the small hydrophobic protein of J paramyxovirus. Journal of virology. 2011 Jan 1;85(1):32-42. https://doi.org/10.1128/JVI.01673-10