Functional and binding studies of HS3.2 of the beta-globin locus control region

Joseph M. Molete, Hanna Petrykowska, Martin Sigg, Webb Miller, Ross Cameron Hardison

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The distal locus control region (LCR) is required for high-level expression of the complex of genes (HBBC) encoding the β-like globins of mammals in erythroid cells. Several major DNase hypersensitive sites (HSs 1-5) mark the LCR. Sequence conservation and direct experimental evidence have implicated sequences within and between the HS cores in function of the LCR. In this report we confirm the mapping of a minor HS between HS3 and HS4, called HS3.2, and show that sequences including it increase the number of random integration sites at which a drug resistance gene is expressed. We also show that nuclear proteins including GATA1 and Oct1 bind specifically to sequences within HS3.2. However, the protein Pbx1, whose binding site is the best match to one highly conserved sequence, does not bind strongly. GATA1 and Oct1 also bind in the HS cores of the LCR and to promoters in HBBC. Their binding to this minor HS suggests that they may be used in assembly of a large complex containing multiple regulatory sequences.

Original languageEnglish (US)
Pages (from-to)185-197
Number of pages13
JournalGene
Volume283
Issue number1-2
DOIs
StatePublished - Jan 23 2002

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Locus Control Region
beta-Globins
Erythroid Cells
Globins
Deoxyribonucleases
Conserved Sequence
Nuclear Proteins
Drug Resistance
Mammals
Binding Sites
Gene Expression
Genes
Proteins

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Molete, Joseph M. ; Petrykowska, Hanna ; Sigg, Martin ; Miller, Webb ; Hardison, Ross Cameron. / Functional and binding studies of HS3.2 of the beta-globin locus control region. In: Gene. 2002 ; Vol. 283, No. 1-2. pp. 185-197.
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Functional and binding studies of HS3.2 of the beta-globin locus control region. / Molete, Joseph M.; Petrykowska, Hanna; Sigg, Martin; Miller, Webb; Hardison, Ross Cameron.

In: Gene, Vol. 283, No. 1-2, 23.01.2002, p. 185-197.

Research output: Contribution to journalArticle

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