Functional and molecular features of the Id4+ germline stem cell population in mouse testes

Frieda Chan, Melissa J. Oatley, Amy V. Kaucher, Qi En Yang, Charles J. Bieberich, Cooduvalli S. Shashikant, Jon M. Oatley

Research output: Contribution to journalArticle

98 Citations (Scopus)

Abstract

The maintenance of cycling cell lineages relies on undifferentiated subpopulations consisting of stem and progenitor pools. Features that delineate these cell types are undefined for many lineages, including spermato-genesis, which is supported by an undifferentiated spermatogonial population. Here, we generated a transgenic mouse line in which spermatogonial stem cells are marked by expression of an inhibitor of differentiation 4 (Id4)-green fluorescent protein (Gfp) transgene. We found that Id4-Gfp+ cells exist primarily as a subset of the type Asingle pool, and their frequency is greatest in neonatal development and then decreases in proportion during establishment of the spermatogenic lineage, eventually comprising ~ 2% of the undifferentiated spermatogonial population in adulthood. RNA sequencing analysis revealed that expression of 11 and 25 genes is unique for the Id4-Gfp+/stem cell and Id4-Gfp-/progenitor fractions, respectively. Collectively, these findings provide the first definitive evidence that stem cells exist as a rare subset of the Asingle pool and reveal transcriptome features distinguishing stem cell and progenitor states within the mammalian male germline.

Original languageEnglish (US)
Pages (from-to)1351-1362
Number of pages12
JournalGenes and Development
Volume28
Issue number12
DOIs
StatePublished - Jun 15 2014

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Green Fluorescent Proteins
Testis
Stem Cells
Population
RNA Sequence Analysis
Cell Lineage
Transgenes
Transcriptome
Transgenic Mice
Cell Differentiation
Maintenance
Genes

All Science Journal Classification (ASJC) codes

  • Genetics
  • Developmental Biology

Cite this

Chan, F., Oatley, M. J., Kaucher, A. V., Yang, Q. E., Bieberich, C. J., Shashikant, C. S., & Oatley, J. M. (2014). Functional and molecular features of the Id4+ germline stem cell population in mouse testes. Genes and Development, 28(12), 1351-1362. https://doi.org/10.1101/gad.240465.114
Chan, Frieda ; Oatley, Melissa J. ; Kaucher, Amy V. ; Yang, Qi En ; Bieberich, Charles J. ; Shashikant, Cooduvalli S. ; Oatley, Jon M. / Functional and molecular features of the Id4+ germline stem cell population in mouse testes. In: Genes and Development. 2014 ; Vol. 28, No. 12. pp. 1351-1362.
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Chan, F, Oatley, MJ, Kaucher, AV, Yang, QE, Bieberich, CJ, Shashikant, CS & Oatley, JM 2014, 'Functional and molecular features of the Id4+ germline stem cell population in mouse testes', Genes and Development, vol. 28, no. 12, pp. 1351-1362. https://doi.org/10.1101/gad.240465.114

Functional and molecular features of the Id4+ germline stem cell population in mouse testes. / Chan, Frieda; Oatley, Melissa J.; Kaucher, Amy V.; Yang, Qi En; Bieberich, Charles J.; Shashikant, Cooduvalli S.; Oatley, Jon M.

In: Genes and Development, Vol. 28, No. 12, 15.06.2014, p. 1351-1362.

Research output: Contribution to journalArticle

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