Functional characterization of Aquaporin-like genes in the human bed bug Cimex lectularius

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The bed bug Cimex lectularius is a blood-feeding re-emerging annoyance pest insect that has the ability to transmit Trypanosoma cruzi under experimental laboratory conditions. Aquaporins (AQPs) are water channel proteins that are essential in biological organisms. C. lectularius are constantly exposed to water-related stress, suggesting that AQPs may offer novel control avenues. We identified and cloned four AQPs from C. lectularius, assessed tissue and lifestage-specific expression, and characterized biochemical functions in vitro and in vivo. We identified an efficient water-specific AQP (ClAQP1), two aquaglyceroporins (ClGlp1 and ClGlp2) and a homolog of Drosophila melanogaster big brain (ClBib). ClGlp1 was only functional when co-expressed with the water-specific AQP. Simultaneous RNAi gene silencing of ClAQP1 and ClGlp1 significantly reduced water and urea excretion post blood feeding. The Bib homologue was enriched in embryos, exclusively expressed in ovaries, and when silenced, dramatically increased bug fecundity. Our data demonstrate that AQPs have critical roles in excretion, water homeostasis and reproduction in C. lectularius, and could be potential targets for control in this notorious pest.

Original languageEnglish (US)
Article number3214
JournalScientific reports
Volume7
Issue number1
DOIs
StatePublished - Dec 1 2017

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Bedbugs
Aquaporins
Genes
Water
Aquaglyceroporins
Trypanosoma cruzi
Gene Silencing
RNA Interference
Drosophila melanogaster
Dehydration
Reproduction
Fertility
Insects
Urea
Ovary
Homeostasis
Embryonic Structures

All Science Journal Classification (ASJC) codes

  • General

Cite this

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title = "Functional characterization of Aquaporin-like genes in the human bed bug Cimex lectularius",
abstract = "The bed bug Cimex lectularius is a blood-feeding re-emerging annoyance pest insect that has the ability to transmit Trypanosoma cruzi under experimental laboratory conditions. Aquaporins (AQPs) are water channel proteins that are essential in biological organisms. C. lectularius are constantly exposed to water-related stress, suggesting that AQPs may offer novel control avenues. We identified and cloned four AQPs from C. lectularius, assessed tissue and lifestage-specific expression, and characterized biochemical functions in vitro and in vivo. We identified an efficient water-specific AQP (ClAQP1), two aquaglyceroporins (ClGlp1 and ClGlp2) and a homolog of Drosophila melanogaster big brain (ClBib). ClGlp1 was only functional when co-expressed with the water-specific AQP. Simultaneous RNAi gene silencing of ClAQP1 and ClGlp1 significantly reduced water and urea excretion post blood feeding. The Bib homologue was enriched in embryos, exclusively expressed in ovaries, and when silenced, dramatically increased bug fecundity. Our data demonstrate that AQPs have critical roles in excretion, water homeostasis and reproduction in C. lectularius, and could be potential targets for control in this notorious pest.",
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Functional characterization of Aquaporin-like genes in the human bed bug Cimex lectularius. / Tsujimoto, Hitoshi; Sakamoto, Joyce Megumi; Rasgon, Jason Laurence.

In: Scientific reports, Vol. 7, No. 1, 3214, 01.12.2017.

Research output: Contribution to journalArticle

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