Functional differentiation of a clone resembling embryonic cortical interneuron progenitors

Hedong Li, Yu R. Han, Caixia Bi, Jonathan Davila, Loyal A. Goff, Kevin Thompson, Mavis Swerdel, Cynthia Camarillo, Christopher L. Ricupero, Ronald P. Hart, Mark R. Plummer, Martin Grumet

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

We have generated clones (L2.3 and RG3.6) of neural progenitors with radial glial properties from rat E14.5 cortex that differentiate into astrocytes, neurons, and oligodendrocytes. Here, we describe a different clone (L2.2) that gives rise exclusively to neurons, but not to glia. Neuronal differentiation of L2.2 cells was inhibited by bone morphogenic protein 2 (BMP2) and enhanced by Sonic Hedgehog (SHH) similar to cortical interneuron progenitors. Compared with L2.3, differentiating L2.2 cells expressed significantly higher levels of mRNAs for glutamate decarboxylases (GADs), DLX transcription factors, calretinin, calbindin, neuropeptide Y (NPY), and somatostatin. Increased levels of DLX-2, GADs, and calretinin proteins were confirmed upon differentiation. L2.2 cells differentiated into neurons that fired action potentials in vitro, and their electrophysiological differentiation was accelerated and more complete when cocultured with developing astroglial cells but not with conditioned medium from these cells. The combined results suggest that clone L2.2 resembles GABAergic interneuron progenitors in the developing forebrain.

Original languageEnglish (US)
Pages (from-to)1549-1564
Number of pages16
JournalDevelopmental Neurobiology
Volume68
Issue number14
DOIs
StatePublished - Dec 2008

All Science Journal Classification (ASJC) codes

  • Developmental Neuroscience
  • Cellular and Molecular Neuroscience

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