Abstract
The NUC-1 regulatory protein directly controls the transcription of genes encoding the phosphorus acquisition enzymes in Neurospora crassa. To understand how NUC-1 regulates the transcription of these genes and how the activity of NUC-1 is modulated by other regulatory proteins, two putative functional domains of NUC-1 were analyzed: the DNA-binding domain and the regulatory domain. The DNA-binding activity of NUC-1 has not been directly demonstrated; however, results of deletion analysis, sequence analysis of the nuc-1 mutant alleles, and strong sequence similarity with the Saccharomyces cerevisiae PHO4 protein strongly suggest that the basic helix-loop-helix motif of NUC-1 forms a DNA-binding domain. Deletion and mutant analyses revealed that 39 amino acid (aa) residues (aa 463 to 501), or fewer, of NUC-1 are interacting with the negative regulatory factor(s), the PREG and/or PGOV proteins.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 259-264 |
| Number of pages | 6 |
| Journal | Gene |
| Volume | 130 |
| Issue number | 2 |
| DOIs | |
| State | Published - Aug 25 1993 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Genetics
Cite this
}
Functional domains of the transcriptional activator NUC-1 in Neurospora crassa. / Kang, Seogchan.
In: Gene, Vol. 130, No. 2, 25.08.1993, p. 259-264.Research output: Contribution to journal › Article
TY - JOUR
T1 - Functional domains of the transcriptional activator NUC-1 in Neurospora crassa
AU - Kang, Seogchan
PY - 1993/8/25
Y1 - 1993/8/25
N2 - The NUC-1 regulatory protein directly controls the transcription of genes encoding the phosphorus acquisition enzymes in Neurospora crassa. To understand how NUC-1 regulates the transcription of these genes and how the activity of NUC-1 is modulated by other regulatory proteins, two putative functional domains of NUC-1 were analyzed: the DNA-binding domain and the regulatory domain. The DNA-binding activity of NUC-1 has not been directly demonstrated; however, results of deletion analysis, sequence analysis of the nuc-1 mutant alleles, and strong sequence similarity with the Saccharomyces cerevisiae PHO4 protein strongly suggest that the basic helix-loop-helix motif of NUC-1 forms a DNA-binding domain. Deletion and mutant analyses revealed that 39 amino acid (aa) residues (aa 463 to 501), or fewer, of NUC-1 are interacting with the negative regulatory factor(s), the PREG and/or PGOV proteins.
AB - The NUC-1 regulatory protein directly controls the transcription of genes encoding the phosphorus acquisition enzymes in Neurospora crassa. To understand how NUC-1 regulates the transcription of these genes and how the activity of NUC-1 is modulated by other regulatory proteins, two putative functional domains of NUC-1 were analyzed: the DNA-binding domain and the regulatory domain. The DNA-binding activity of NUC-1 has not been directly demonstrated; however, results of deletion analysis, sequence analysis of the nuc-1 mutant alleles, and strong sequence similarity with the Saccharomyces cerevisiae PHO4 protein strongly suggest that the basic helix-loop-helix motif of NUC-1 forms a DNA-binding domain. Deletion and mutant analyses revealed that 39 amino acid (aa) residues (aa 463 to 501), or fewer, of NUC-1 are interacting with the negative regulatory factor(s), the PREG and/or PGOV proteins.
UR - http://www.scopus.com/inward/record.url?scp=0027219676&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027219676&partnerID=8YFLogxK
U2 - 10.1016/0378-1119(93)90428-6
DO - 10.1016/0378-1119(93)90428-6
M3 - Article
C2 - 8359693
AN - SCOPUS:0027219676
VL - 130
SP - 259
EP - 264
JO - Gene
JF - Gene
SN - 0378-1119
IS - 2
ER -