Functional genomic analysis in pain research using hybridization arrays.

Stephen J. Walker, Travis J. Worst, Willard M. Freeman, Kent Vrana

Research output: Contribution to journalArticle

Abstract

Hybridization array technology makes it possible to compare global gene-expression patterns in any experimental context for which good-quality RNA can be generated. To date, DNA arrays have been used as a tool to compare functional genomic changes (differences in wholesale gene expression) in studies that cover an impressive variety of research disciplines including cancer, yeast genomics, and, more recently, neuroscience and behavior. The basic premise of the array experiment is that one interrogates a panel of probes (gene-specific cDNA fragments or gene-specific oligonucleotides that have been immobilized on a solid support) with RNAs (targets) from control and treated experimental samples that have been either radioactively or fluorescently labeled. Signal derived from either competitive (both samples on a single chip) or differential (one sample/one chip) hybridization is used to calculate relative gene expression. There are three widely used platforms available to perform array experiments (Affymetrix GeneChips, oligonucleotide arrays, and membrane-based cDNA arrays) and each platform offers advantages and limitations. The experimental description in this chapter explains, in detail, how to perform a hybridization array using the macroarray platform.

Original languageEnglish (US)
Pages (from-to)239-253
Number of pages15
JournalMethods in molecular medicine
Volume99
StatePublished - Jan 1 2004

Fingerprint

Oligonucleotide Array Sequence Analysis
Gene Expression
Pain
Research
RNA
Neurosciences
Genomics
Oligonucleotides
Genes
Complementary DNA
Yeasts
Technology
Membranes
Neoplasms

All Science Journal Classification (ASJC) codes

  • Molecular Medicine

Cite this

Walker, Stephen J. ; Worst, Travis J. ; Freeman, Willard M. ; Vrana, Kent. / Functional genomic analysis in pain research using hybridization arrays. In: Methods in molecular medicine. 2004 ; Vol. 99. pp. 239-253.
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Functional genomic analysis in pain research using hybridization arrays. / Walker, Stephen J.; Worst, Travis J.; Freeman, Willard M.; Vrana, Kent.

In: Methods in molecular medicine, Vol. 99, 01.01.2004, p. 239-253.

Research output: Contribution to journalArticle

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