Functional homology of mammalian and yeast RAS genes

Tohru Kataoka, Scott Powers, Scott Cameron, Ottavio Fasano, Mitchell Goldfarb, James Broach, Michael Wigler

Research output: Contribution to journalArticle

160 Scopus citations

Abstract

Yeast spores lacking endogenous RAS genes will not germinate. If such spores contain chimeric mammalian/yeast RAS genes or even the mammalian H-ras gene under the control of the galactose-inducible GAL10 promoter, they will germinate in the presence of galactose and produce viable haploid progeny dependent on galactose for continued growth and viability. These results indicate that the biochemical function of RAS proteins is essential for vegetative haploid yeast and that this function has been conserved in evolution since the progenitors of yeast and mammals diverged.

Original languageEnglish (US)
Pages (from-to)19-26
Number of pages8
JournalCell
Volume40
Issue number1
DOIs
StatePublished - Jan 1985

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

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    Kataoka, T., Powers, S., Cameron, S., Fasano, O., Goldfarb, M., Broach, J., & Wigler, M. (1985). Functional homology of mammalian and yeast RAS genes. Cell, 40(1), 19-26. https://doi.org/10.1016/0092-8674(85)90304-6