Functional homology of mammalian and yeast RAS genes

Tohru Kataoka; Scott Powers; Scott Cameron; Ottavio Fasano; Mitchell Goldfarb; James Broach; Michael Wigler

doi:10.1016/0092-8674(85)90304-6

Functional homology of mammalian and yeast RAS genes

Tohru Kataoka, Scott Powers, Scott Cameron, Ottavio Fasano, Mitchell Goldfarb, James Broach, Michael Wigler

Research output: Contribution to journal › Article › peer-review

171 Scopus citations

Abstract

Yeast spores lacking endogenous RAS genes will not germinate. If such spores contain chimeric mammalian/yeast RAS genes or even the mammalian H-ras gene under the control of the galactose-inducible GAL10 promoter, they will germinate in the presence of galactose and produce viable haploid progeny dependent on galactose for continued growth and viability. These results indicate that the biochemical function of RAS proteins is essential for vegetative haploid yeast and that this function has been conserved in evolution since the progenitors of yeast and mammals diverged.

Original language	English (US)
Pages (from-to)	19-26
Number of pages	8
Journal	Cell
Volume	40
Issue number	1
DOIs	https://doi.org/10.1016/0092-8674(85)90304-6
State	Published - Jan 1985

All Science Journal Classification (ASJC) codes

Biochemistry, Genetics and Molecular Biology(all)

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10.1016/0092-8674(85)90304-6

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title = "Functional homology of mammalian and yeast RAS genes",

abstract = "Yeast spores lacking endogenous RAS genes will not germinate. If such spores contain chimeric mammalian/yeast RAS genes or even the mammalian H-ras gene under the control of the galactose-inducible GAL10 promoter, they will germinate in the presence of galactose and produce viable haploid progeny dependent on galactose for continued growth and viability. These results indicate that the biochemical function of RAS proteins is essential for vegetative haploid yeast and that this function has been conserved in evolution since the progenitors of yeast and mammals diverged.",

author = "Tohru Kataoka and Scott Powers and Scott Cameron and Ottavio Fasano and Mitchell Goldfarb and James Broach and Michael Wigler",

note = "Funding Information: This work w s supported by gr nts from the N tion l Institutes of He lth nd the Americ n Business for C ncer Rese rch Found tion . S . P. is postdoctor l fellow of the Leukemi Society of Americ . J . B . is n Est blished Investig tor of the Americ n He rt Associ tion . T. K . is on le ve from the Dep rtment of Genetics, Os k University Medic l School, J p n . O. F. is on le ve from I Istituto di Chimic Biologic , II F colt di Medicin , Universit di N poli, It ly.",

year = "1985",

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doi = "10.1016/0092-8674(85)90304-6",

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T1 - Functional homology of mammalian and yeast RAS genes

AU - Kataoka, Tohru

AU - Powers, Scott

AU - Cameron, Scott

AU - Fasano, Ottavio

AU - Goldfarb, Mitchell

AU - Broach, James

AU - Wigler, Michael

N1 - Funding Information: This work w s supported by gr nts from the N tion l Institutes of He lth nd the Americ n Business for C ncer Rese rch Found tion . S . P. is postdoctor l fellow of the Leukemi Society of Americ . J . B . is n Est blished Investig tor of the Americ n He rt Associ tion . T. K . is on le ve from the Dep rtment of Genetics, Os k University Medic l School, J p n . O. F. is on le ve from I Istituto di Chimic Biologic , II F colt di Medicin , Universit di N poli, It ly.

PY - 1985/1

Y1 - 1985/1

N2 - Yeast spores lacking endogenous RAS genes will not germinate. If such spores contain chimeric mammalian/yeast RAS genes or even the mammalian H-ras gene under the control of the galactose-inducible GAL10 promoter, they will germinate in the presence of galactose and produce viable haploid progeny dependent on galactose for continued growth and viability. These results indicate that the biochemical function of RAS proteins is essential for vegetative haploid yeast and that this function has been conserved in evolution since the progenitors of yeast and mammals diverged.

AB - Yeast spores lacking endogenous RAS genes will not germinate. If such spores contain chimeric mammalian/yeast RAS genes or even the mammalian H-ras gene under the control of the galactose-inducible GAL10 promoter, they will germinate in the presence of galactose and produce viable haploid progeny dependent on galactose for continued growth and viability. These results indicate that the biochemical function of RAS proteins is essential for vegetative haploid yeast and that this function has been conserved in evolution since the progenitors of yeast and mammals diverged.

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Functional homology of mammalian and yeast RAS genes

Abstract

All Science Journal Classification (ASJC) codes

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