Functional identification of the general acid and base in the dehydration step of indole-3-glycerol phosphate synthase catalysis

Margot J. Zaccardi, Eric M. Yezdimer, David D. Boehr

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Background: Bacteria require the enzyme indole-3-glycerol phosphate synthase for the production of Trp. Results: Glu-51 and Lys-53 are identified as the base and acid acting in the dehydration step of enzyme catalysis. Conclusion: Ring closure and dehydration steps are catalyzed by distinct active-site surfaces. Significance: Enzyme inhibitors targeted against these active-site surfaces may serve as novel antibiotics.

Original languageEnglish (US)
Pages (from-to)26350-26356
Number of pages7
JournalJournal of Biological Chemistry
Volume288
Issue number37
DOIs
StatePublished - Sep 13 2013

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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