Functional localization of a melanoma tumor suppressor gene to a small (≤ 2 Mb) region on 11q23

Gavin Robertson, Eleonora K. Goldberg, Tracy G. Lugo, Jane W. Fountain

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

We have previously demonstrated the existence of a melanoma tumor suppressor gene(s) on the long arm of chromosome 11 through suppression of tumorigenicity assays. Although loss of heterozygosity studies also support this finding, only a large critical region (44 cM) has been identified to date on 11q22-25. To further localize a tumor suppressor gene(s) within this region, we have now generated and characterized nine melanoma microcell hybrids, each retaining an introduced fragment of 11q. Of the nine hybrids, four were suppressed for tumor formation in nude mice, while five formed tumors at the same rate as the parental melanoma cell line (UACC 903). Molecular analysis of the hybrids with 118 microsatellite markers narrowed the location of a putative suppressor gene to a small (≤ 2 Mb) candidate region on 11q23 between the markers D11S1786 and D11S2077 and within the larger region frequently deleted in melanoma tumors and cell lines. While multiple tumor suppressor genes are likely to reside on 11q22-25, the presence of this region in all four suppressed hybrids supports the simplest model that a single locus is responsible for the suppressed phenotype observed in UACC 903.

Original languageEnglish (US)
Pages (from-to)3173-3180
Number of pages8
JournalOncogene
Volume18
Issue number20
DOIs
StatePublished - May 20 1999

Fingerprint

Tumor Suppressor Genes
Melanoma
Suppressor Genes
Chromosomes, Human, Pair 11
Loss of Heterozygosity
Tumor Cell Line
Nude Mice
Microsatellite Repeats
Neoplasms
Phenotype
Cell Line

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this

Robertson, Gavin ; Goldberg, Eleonora K. ; Lugo, Tracy G. ; Fountain, Jane W. / Functional localization of a melanoma tumor suppressor gene to a small (≤ 2 Mb) region on 11q23. In: Oncogene. 1999 ; Vol. 18, No. 20. pp. 3173-3180.
@article{ee5610de76b74279b92fc72146d90958,
title = "Functional localization of a melanoma tumor suppressor gene to a small (≤ 2 Mb) region on 11q23",
abstract = "We have previously demonstrated the existence of a melanoma tumor suppressor gene(s) on the long arm of chromosome 11 through suppression of tumorigenicity assays. Although loss of heterozygosity studies also support this finding, only a large critical region (44 cM) has been identified to date on 11q22-25. To further localize a tumor suppressor gene(s) within this region, we have now generated and characterized nine melanoma microcell hybrids, each retaining an introduced fragment of 11q. Of the nine hybrids, four were suppressed for tumor formation in nude mice, while five formed tumors at the same rate as the parental melanoma cell line (UACC 903). Molecular analysis of the hybrids with 118 microsatellite markers narrowed the location of a putative suppressor gene to a small (≤ 2 Mb) candidate region on 11q23 between the markers D11S1786 and D11S2077 and within the larger region frequently deleted in melanoma tumors and cell lines. While multiple tumor suppressor genes are likely to reside on 11q22-25, the presence of this region in all four suppressed hybrids supports the simplest model that a single locus is responsible for the suppressed phenotype observed in UACC 903.",
author = "Gavin Robertson and Goldberg, {Eleonora K.} and Lugo, {Tracy G.} and Fountain, {Jane W.}",
year = "1999",
month = "5",
day = "20",
doi = "10.1038/sj.onc.1202664",
language = "English (US)",
volume = "18",
pages = "3173--3180",
journal = "Oncogene",
issn = "0950-9232",
publisher = "Nature Publishing Group",
number = "20",

}

Functional localization of a melanoma tumor suppressor gene to a small (≤ 2 Mb) region on 11q23. / Robertson, Gavin; Goldberg, Eleonora K.; Lugo, Tracy G.; Fountain, Jane W.

In: Oncogene, Vol. 18, No. 20, 20.05.1999, p. 3173-3180.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Functional localization of a melanoma tumor suppressor gene to a small (≤ 2 Mb) region on 11q23

AU - Robertson, Gavin

AU - Goldberg, Eleonora K.

AU - Lugo, Tracy G.

AU - Fountain, Jane W.

PY - 1999/5/20

Y1 - 1999/5/20

N2 - We have previously demonstrated the existence of a melanoma tumor suppressor gene(s) on the long arm of chromosome 11 through suppression of tumorigenicity assays. Although loss of heterozygosity studies also support this finding, only a large critical region (44 cM) has been identified to date on 11q22-25. To further localize a tumor suppressor gene(s) within this region, we have now generated and characterized nine melanoma microcell hybrids, each retaining an introduced fragment of 11q. Of the nine hybrids, four were suppressed for tumor formation in nude mice, while five formed tumors at the same rate as the parental melanoma cell line (UACC 903). Molecular analysis of the hybrids with 118 microsatellite markers narrowed the location of a putative suppressor gene to a small (≤ 2 Mb) candidate region on 11q23 between the markers D11S1786 and D11S2077 and within the larger region frequently deleted in melanoma tumors and cell lines. While multiple tumor suppressor genes are likely to reside on 11q22-25, the presence of this region in all four suppressed hybrids supports the simplest model that a single locus is responsible for the suppressed phenotype observed in UACC 903.

AB - We have previously demonstrated the existence of a melanoma tumor suppressor gene(s) on the long arm of chromosome 11 through suppression of tumorigenicity assays. Although loss of heterozygosity studies also support this finding, only a large critical region (44 cM) has been identified to date on 11q22-25. To further localize a tumor suppressor gene(s) within this region, we have now generated and characterized nine melanoma microcell hybrids, each retaining an introduced fragment of 11q. Of the nine hybrids, four were suppressed for tumor formation in nude mice, while five formed tumors at the same rate as the parental melanoma cell line (UACC 903). Molecular analysis of the hybrids with 118 microsatellite markers narrowed the location of a putative suppressor gene to a small (≤ 2 Mb) candidate region on 11q23 between the markers D11S1786 and D11S2077 and within the larger region frequently deleted in melanoma tumors and cell lines. While multiple tumor suppressor genes are likely to reside on 11q22-25, the presence of this region in all four suppressed hybrids supports the simplest model that a single locus is responsible for the suppressed phenotype observed in UACC 903.

UR - http://www.scopus.com/inward/record.url?scp=0033587013&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033587013&partnerID=8YFLogxK

U2 - 10.1038/sj.onc.1202664

DO - 10.1038/sj.onc.1202664

M3 - Article

C2 - 10340390

AN - SCOPUS:0033587013

VL - 18

SP - 3173

EP - 3180

JO - Oncogene

JF - Oncogene

SN - 0950-9232

IS - 20

ER -