Functional roles of the pre-sensor I insertion sequence in an AAA+ bacterial enhancer binding protein

Patricia C. Burrows, Jörg Schumacher, Samuel Amartey, Tamaswati Ghosh, Timothy A. Burgis, Xiaodong Zhang, B. Tracy Nixon, Martin Buck

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Molecular machines belonging to the AAA+ superfamily of ATPases use NTP hydrolysis to remodel their versatile substrates. The presence of an insertion sequence defines the major phylogenetic presensor I insertion (pre-Sli) AAA+ superclade. In the bacterial σ54-dependent enhancer binding protein phage shock protein F (PspF) the pre-Sli loop adopts different conformations depending on the nucleotidebound state. Single amino acid substitutions within the dynamic pre-Sli loop of PspF drastically change the ATP hydrolysis parameters, indicating a structural link to the distant hydrolysis site. We used a sitespecific protein-DNA proximity assay to measure the contribution of the pre-Sli loop in σ54-dependent transcription and demonstrate that the pre-Sli loop is a major structural feature mediating nucleotide statedependent differential engagement with Eσ54. We suggest that much, if not all, of the action of the pre-Sli loop is mediated through the L1 loop and relies on a conserved molecular switch, identified in a crystal structure of one pre-Sli variant and in accordance with the high covariance between some pre-Sli residues and distinct residues outside the pre-Sli sequence.

Original languageEnglish (US)
Pages (from-to)519-533
Number of pages15
JournalMolecular Microbiology
Volume73
Issue number4
DOIs
StatePublished - Aug 1 2009

Fingerprint

Insertional Mutagenesis
Carrier Proteins
Hydrolysis
Bacteriophages
Shock
Proteins
Amino Acid Substitution
Adenosine Triphosphatases
Nucleotides
Adenosine Triphosphate
DNA

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Cite this

Burrows, P. C., Schumacher, J., Amartey, S., Ghosh, T., Burgis, T. A., Zhang, X., ... Buck, M. (2009). Functional roles of the pre-sensor I insertion sequence in an AAA+ bacterial enhancer binding protein. Molecular Microbiology, 73(4), 519-533. https://doi.org/10.1111/j.1365-2958.2009.06744.x
Burrows, Patricia C. ; Schumacher, Jörg ; Amartey, Samuel ; Ghosh, Tamaswati ; Burgis, Timothy A. ; Zhang, Xiaodong ; Nixon, B. Tracy ; Buck, Martin. / Functional roles of the pre-sensor I insertion sequence in an AAA+ bacterial enhancer binding protein. In: Molecular Microbiology. 2009 ; Vol. 73, No. 4. pp. 519-533.
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Burrows, PC, Schumacher, J, Amartey, S, Ghosh, T, Burgis, TA, Zhang, X, Nixon, BT & Buck, M 2009, 'Functional roles of the pre-sensor I insertion sequence in an AAA+ bacterial enhancer binding protein', Molecular Microbiology, vol. 73, no. 4, pp. 519-533. https://doi.org/10.1111/j.1365-2958.2009.06744.x

Functional roles of the pre-sensor I insertion sequence in an AAA+ bacterial enhancer binding protein. / Burrows, Patricia C.; Schumacher, Jörg; Amartey, Samuel; Ghosh, Tamaswati; Burgis, Timothy A.; Zhang, Xiaodong; Nixon, B. Tracy; Buck, Martin.

In: Molecular Microbiology, Vol. 73, No. 4, 01.08.2009, p. 519-533.

Research output: Contribution to journalArticle

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