Functional stability of a mixed microbial consortium producing PHA from waste carbon sources

Erik R. Coats, Frank J. Loge, William A. Smith, David N. Thompson, Michael P Wolcott

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Polyhydroxyalkanoates (PHAs) represent an environmentally effective alternative to synthetic thermoplastics; however, current production practices are not sustainable. In this study, PHA production was accomplished in sequencing batch bioreactors utilizing real wastewaters and mixed microbial consortia from municipal activated sludge as inoculum. Polymer production reached 85, 53, and 10% of the cell dry weight from methanol-enriched pulp and paper mill foul condensate, fermented municipal primary solids, and biodiesel wastewater, respectively. Using denaturing gradient gel electrophoresis of 16S-rDNA from polymerase chain reaction-amplified DNA extracts, distinctly different communities were observed between and within wastewaters following enrichment. Most importantly, functional stability was maintained despite differing and contrasting microbial populations.

Original languageEnglish (US)
Pages (from-to)909-925
Number of pages17
JournalApplied Biochemistry and Biotechnology
Volume137-140
Issue number1-12
DOIs
StatePublished - Apr 1 2007

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Microbial Consortia
Polyhydroxyalkanoates
Waste Water
Wastewater
Carbon
Denaturing Gradient Gel Electrophoresis
Biofuels
Polymerase chain reaction
Paper and pulp mills
Bioreactors
Biodiesel
Sewage
Ribosomal DNA
Electrophoresis
Thermoplastics
Methanol
Polymers
DNA
Gels
Weights and Measures

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology

Cite this

Coats, Erik R. ; Loge, Frank J. ; Smith, William A. ; Thompson, David N. ; Wolcott, Michael P. / Functional stability of a mixed microbial consortium producing PHA from waste carbon sources. In: Applied Biochemistry and Biotechnology. 2007 ; Vol. 137-140, No. 1-12. pp. 909-925.
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Functional stability of a mixed microbial consortium producing PHA from waste carbon sources. / Coats, Erik R.; Loge, Frank J.; Smith, William A.; Thompson, David N.; Wolcott, Michael P.

In: Applied Biochemistry and Biotechnology, Vol. 137-140, No. 1-12, 01.04.2007, p. 909-925.

Research output: Contribution to journalArticle

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