Fundamentals of affinity cell separations

Ye Zhang, Veronica Lyons, Dimitri Pappas

Research output: Contribution to journalReview article

7 Scopus citations

Abstract

Cell separations using affinity methods continue to be an enabling science for a wide variety of applications. In this review, we discuss the fundamental aspects of affinity separation, including the competing forces for cell capture and elution, cell-surface interactions, and models for cell adhesion. Factors affecting separation performance such as bond affinity, contact area, and temperature are presented. We also discuss and demonstrate the effects of nonspecific binding on separation performance. Metrics for evaluating cell separations are presented, along with methods of comparing separation techniques for cell isolation using affinity capture.

Original languageEnglish (US)
Pages (from-to)732-741
Number of pages10
JournalELECTROPHORESIS
Volume39
Issue number5-6
DOIs
StatePublished - Mar 1 2018

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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  • Cite this

    Zhang, Y., Lyons, V., & Pappas, D. (2018). Fundamentals of affinity cell separations. ELECTROPHORESIS, 39(5-6), 732-741. https://doi.org/10.1002/elps.201700311