Fungal pretreatment for non-sterile Miscanthus × giganteus for enhanced sugar yield in enzymatic hydrolysis

Juliana Vasco-Correa, Xumeng Ge, Yebo Li

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Miscanthus is an energy crop with high potential for the biofuels industry, because of its high annual biomass yields, low nutrients and water requirements, and remarkable adaptability to different climate and soil conditions. Since the lignocellulosic structure of miscanthus is recalcitrant to digest, pretreatment is needed to enhance sugar release during the sequential enzymatic hydrolysis. Commonly used thermochemical pretreatment methods operate under harsh conditions and have a large water footprint. In contrast, fungal pretreatment can be performed under mild conditions, using minimal amounts of chemicals and water. However, a costly feedstock sterilization step is generally required prior to fungal pretreatment, in order to eliminate negative impacts of indigenous microorganisms. Using pre-colonized feedstock as the inoculum is a promising strategy to address this issue, but has not been studied in fungal pretreatment of grassy biomass, such as miscanthus. In this study, fungal pretreatment of non-sterile Miscanthus × giganteous with Ceriporiopsis subvermispora was conducted, using previously colonized miscanthus as an inoculum. Five different inoculum ratios (10, 20, 30, 40, and 50%), and two moisture contents (60 and 75%) were tested. Results showed that inoculum ratios equal or greater than 30% enhanced the enzymatic digestibility of miscanthus 3-4-fold, which was consistent with the lignin degradation during pretreatment. Sugar loss up to 24% during fungal pretreatment was observed, but the final sugar yield could still be improved 2.5-4-fold.

Original languageEnglish (US)
Title of host publicationAmerican Society of Agricultural and Biological Engineers Annual International Meeting 2015
PublisherAmerican Society of Agricultural and Biological Engineers
Pages4172-4178
Number of pages7
ISBN (Electronic)9781510810501
DOIs
StatePublished - Jan 1 2015
EventAmerican Society of Agricultural and Biological Engineers Annual International Meeting 2015 - New Orleans, United States
Duration: Jul 26 2015Jul 29 2015

Publication series

NameAmerican Society of Agricultural and Biological Engineers Annual International Meeting 2015
Volume5

Other

OtherAmerican Society of Agricultural and Biological Engineers Annual International Meeting 2015
CountryUnited States
CityNew Orleans
Period7/26/157/29/15

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Agronomy and Crop Science

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    Vasco-Correa, J., Ge, X., & Li, Y. (2015). Fungal pretreatment for non-sterile Miscanthus × giganteus for enhanced sugar yield in enzymatic hydrolysis. In American Society of Agricultural and Biological Engineers Annual International Meeting 2015 (pp. 4172-4178). (American Society of Agricultural and Biological Engineers Annual International Meeting 2015; Vol. 5). American Society of Agricultural and Biological Engineers. https://doi.org/10.13031/aim.20152189948