FUSARIUM-ID v. 1.0: A DNA sequence database for identifying Fusarium

David M. Geiser, María Del Mar Jiménez-Gasco, Seogchan Kang, Izabela Makalowska, Narayanan Veeraraghavan, Todd J. Ward, Ning Zhang, Gretchen A. Kuldau, Kerry O'Donnell

Research output: Contribution to journalArticle

576 Citations (Scopus)

Abstract

One of the greatest impediments to the study of Fusarium has been the incorrect and confused application of species names to toxigenic and pathogenic isolates, owing in large part to intrinsic limitations of morphological species recognition and its application. To address this problem, we have created FUSARIUM-ID v. 1.0, a publicly available database of partial translation elongation factor 1-alpha (TEF) DNA sequences, presently representing a selected sample of the diversity of the genus diversity, with excellent representation of Type-B trichothecene toxin producers, and the Gibberella fujikuroi, Fusarium oxysporum and F. solani species complexes. Users can generate sequences using primers that are conserved across the genus, and use the sequence as a query to BLAST the database, which can be accessed at http://fusarium.cbio.psu.edu, or in a phylogenetic analysis. Correct identification of a known species in these groups often can be performed using this gene region alone. This growing database will contain only vouchered sequences attached to publicly available cultures. In the future, FUSARIUM-ID will be expanded to include additional sequences, including multiple sequences from the same species, sequences from new and revised species, and information from additional genes.

Original languageEnglish (US)
Pages (from-to)473-479
Number of pages7
JournalEuropean Journal of Plant Pathology
Volume110
Issue number5-6
DOIs
StatePublished - Jun 1 2004

Fingerprint

Fusarium
nucleotide sequences
trichothecenes
Fusarium solani
Fusarium oxysporum
translation (genetics)
toxins
genes
phylogeny
sampling
species identification

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Plant Science
  • Horticulture

Cite this

Geiser, David M. ; Jiménez-Gasco, María Del Mar ; Kang, Seogchan ; Makalowska, Izabela ; Veeraraghavan, Narayanan ; Ward, Todd J. ; Zhang, Ning ; Kuldau, Gretchen A. ; O'Donnell, Kerry. / FUSARIUM-ID v. 1.0 : A DNA sequence database for identifying Fusarium. In: European Journal of Plant Pathology. 2004 ; Vol. 110, No. 5-6. pp. 473-479.
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FUSARIUM-ID v. 1.0 : A DNA sequence database for identifying Fusarium. / Geiser, David M.; Jiménez-Gasco, María Del Mar; Kang, Seogchan; Makalowska, Izabela; Veeraraghavan, Narayanan; Ward, Todd J.; Zhang, Ning; Kuldau, Gretchen A.; O'Donnell, Kerry.

In: European Journal of Plant Pathology, Vol. 110, No. 5-6, 01.06.2004, p. 473-479.

Research output: Contribution to journalArticle

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AU - Ward, Todd J.

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