Abstract
One of the greatest impediments to the study of Fusarium has been the incorrect and confused application of species names to toxigenic and pathogenic isolates, owing in large part to intrinsic limitations of morphological species recognition and its application. To address this problem, we have created FUSARIUM-ID v. 1.0, a publicly available database of partial translation elongation factor 1-alpha (TEF) DNA sequences, presently representing a selected sample of the diversity of the genus diversity, with excellent representation of Type-B trichothecene toxin producers, and the Gibberella fujikuroi, Fusarium oxysporum and F. solani species complexes. Users can generate sequences using primers that are conserved across the genus, and use the sequence as a query to BLAST the database, which can be accessed at http://fusarium.cbio.psu.edu, or in a phylogenetic analysis. Correct identification of a known species in these groups often can be performed using this gene region alone. This growing database will contain only vouchered sequences attached to publicly available cultures. In the future, FUSARIUM-ID will be expanded to include additional sequences, including multiple sequences from the same species, sequences from new and revised species, and information from additional genes.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 473-479 |
| Number of pages | 7 |
| Journal | European Journal of Plant Pathology |
| Volume | 110 |
| Issue number | 5-6 |
| DOIs | |
| State | Published - Jun 1 2004 |
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All Science Journal Classification (ASJC) codes
- Agronomy and Crop Science
- Plant Science
- Horticulture
Cite this
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FUSARIUM-ID v. 1.0 : A DNA sequence database for identifying Fusarium. / Geiser, David M.; Jiménez-Gasco, María Del Mar; Kang, Seogchan; Makalowska, Izabela; Veeraraghavan, Narayanan; Ward, Todd J.; Zhang, Ning; Kuldau, Gretchen A.; O'Donnell, Kerry.
In: European Journal of Plant Pathology, Vol. 110, No. 5-6, 01.06.2004, p. 473-479.Research output: Contribution to journal › Article
TY - JOUR
T1 - FUSARIUM-ID v. 1.0
T2 - A DNA sequence database for identifying Fusarium
AU - Geiser, David M.
AU - Jiménez-Gasco, María Del Mar
AU - Kang, Seogchan
AU - Makalowska, Izabela
AU - Veeraraghavan, Narayanan
AU - Ward, Todd J.
AU - Zhang, Ning
AU - Kuldau, Gretchen A.
AU - O'Donnell, Kerry
PY - 2004/6/1
Y1 - 2004/6/1
N2 - One of the greatest impediments to the study of Fusarium has been the incorrect and confused application of species names to toxigenic and pathogenic isolates, owing in large part to intrinsic limitations of morphological species recognition and its application. To address this problem, we have created FUSARIUM-ID v. 1.0, a publicly available database of partial translation elongation factor 1-alpha (TEF) DNA sequences, presently representing a selected sample of the diversity of the genus diversity, with excellent representation of Type-B trichothecene toxin producers, and the Gibberella fujikuroi, Fusarium oxysporum and F. solani species complexes. Users can generate sequences using primers that are conserved across the genus, and use the sequence as a query to BLAST the database, which can be accessed at http://fusarium.cbio.psu.edu, or in a phylogenetic analysis. Correct identification of a known species in these groups often can be performed using this gene region alone. This growing database will contain only vouchered sequences attached to publicly available cultures. In the future, FUSARIUM-ID will be expanded to include additional sequences, including multiple sequences from the same species, sequences from new and revised species, and information from additional genes.
AB - One of the greatest impediments to the study of Fusarium has been the incorrect and confused application of species names to toxigenic and pathogenic isolates, owing in large part to intrinsic limitations of morphological species recognition and its application. To address this problem, we have created FUSARIUM-ID v. 1.0, a publicly available database of partial translation elongation factor 1-alpha (TEF) DNA sequences, presently representing a selected sample of the diversity of the genus diversity, with excellent representation of Type-B trichothecene toxin producers, and the Gibberella fujikuroi, Fusarium oxysporum and F. solani species complexes. Users can generate sequences using primers that are conserved across the genus, and use the sequence as a query to BLAST the database, which can be accessed at http://fusarium.cbio.psu.edu, or in a phylogenetic analysis. Correct identification of a known species in these groups often can be performed using this gene region alone. This growing database will contain only vouchered sequences attached to publicly available cultures. In the future, FUSARIUM-ID will be expanded to include additional sequences, including multiple sequences from the same species, sequences from new and revised species, and information from additional genes.
UR - http://www.scopus.com/inward/record.url?scp=3442882259&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=3442882259&partnerID=8YFLogxK
U2 - 10.1023/B:EJPP.0000032386.75915.a0
DO - 10.1023/B:EJPP.0000032386.75915.a0
M3 - Article
AN - SCOPUS:3442882259
VL - 110
SP - 473
EP - 479
JO - European Journal of Plant Pathology
JF - European Journal of Plant Pathology
SN - 0929-1873
IS - 5-6
ER -