Fusarium oxysporum infection assays in arabidopsis

Yuri Trusov, David Chakravorty, Jose Ramon Botella

Research output: Chapter in Book/Report/Conference proceedingChapter

6 Citations (Scopus)

Abstract

Increased susceptibility to Fusarium oxysporum is one of the most conspicuous characteristics of the Arabidopsis mutants lacking the heterotrimeric G protein β and γ1 subunits. The molecular mechanisms placing these G proteins in the plant innate immunity network are yet to be discovered. However, a method to test susceptibility to and disease progression of an important plant pathogen, such as F. oxysporum, is of central importance to many plant defense studies. The optimized protocol presented here allows the routine processing and analysis of symptom progression in young Arabidopsis soil-grown seedlings and yields highly reproducible results.

Original languageEnglish (US)
Title of host publicationG Protein-Coupled Receptor Signaling in Plants
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages67-72
Number of pages6
ISBN (Print)9781627035316
DOIs
StatePublished - Jan 1 2013

Publication series

NameMethods in Molecular Biology
Volume1043
ISSN (Print)1064-3745

Fingerprint

Fusariosis
Arabidopsis
Plant Immunity
Heterotrimeric GTP-Binding Proteins
Protein Subunits
Fusarium
Seedlings
GTP-Binding Proteins
Innate Immunity
Disease Progression
Soil

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

Trusov, Y., Chakravorty, D., & Botella, J. R. (2013). Fusarium oxysporum infection assays in arabidopsis. In G Protein-Coupled Receptor Signaling in Plants: Methods and Protocols (pp. 67-72). (Methods in Molecular Biology; Vol. 1043). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-532-3_7
Trusov, Yuri ; Chakravorty, David ; Botella, Jose Ramon. / Fusarium oxysporum infection assays in arabidopsis. G Protein-Coupled Receptor Signaling in Plants: Methods and Protocols. Humana Press Inc., 2013. pp. 67-72 (Methods in Molecular Biology).
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Trusov, Y, Chakravorty, D & Botella, JR 2013, Fusarium oxysporum infection assays in arabidopsis. in G Protein-Coupled Receptor Signaling in Plants: Methods and Protocols. Methods in Molecular Biology, vol. 1043, Humana Press Inc., pp. 67-72. https://doi.org/10.1007/978-1-62703-532-3_7

Fusarium oxysporum infection assays in arabidopsis. / Trusov, Yuri; Chakravorty, David; Botella, Jose Ramon.

G Protein-Coupled Receptor Signaling in Plants: Methods and Protocols. Humana Press Inc., 2013. p. 67-72 (Methods in Molecular Biology; Vol. 1043).

Research output: Chapter in Book/Report/Conference proceedingChapter

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Trusov Y, Chakravorty D, Botella JR. Fusarium oxysporum infection assays in arabidopsis. In G Protein-Coupled Receptor Signaling in Plants: Methods and Protocols. Humana Press Inc. 2013. p. 67-72. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-62703-532-3_7