Fusion between transcription factor CBFβ/PEBP2β and a myosin heavy chain in acute myeloid leukemia

Pu Liu, Susan A. Tarlé, Amitav Hajra, David Claxton, Paula Marlton, Matthew Freedman, Michael J. Siciliano, Francis S. Collins

Research output: Contribution to journalArticle

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Abstract

The pericentric inversion of chromosome 16 [inv(16)(p13q22)] is a characteristic karyotypic abnormality associated with acute myeloid leukemia, most commonly of the M4Eo subtype. The 16p and 16q breakpoints were pinpointed by yeast artificial chromosome and cosmid cloning, and the two genes involved in this inversion were identified. On 16q the inversion occurred near the end of the coding region for CBFβ, also known as PEBP2β, a subunit of a heterodimeric transcription factor regulating genes expressed in T cells; on 16p a smooth muscle myosin heavy chain (SMMHC) gene (MYH11) was interrupted. In six of six inv(16) patient samples tested, an in-frame fusion messenger RNA was demonstrated that connected the first 165 amino acids of CBFβ with the tail region of SMMHC. The repeated coiled coil of SMMHC may result in dimerization of the CBFβ fusion protein, which in turn would lead to alterations in transcriptional regulation and contribute to leukemic transformation.

Original languageEnglish (US)
Pages (from-to)1041-1044
Number of pages4
JournalScience
Volume261
Issue number5124
DOIs
StatePublished - Jan 1 1993

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Core Binding Factors
Smooth Muscle Myosins
Myosin Heavy Chains
Acute Myeloid Leukemia
Yeast Artificial Chromosomes
Genes
Chromosomes, Human, Pair 16
Cosmids
Dimerization
Organism Cloning
Transcription Factors
T-Lymphocytes
Amino Acids
Messenger RNA
Proteins

All Science Journal Classification (ASJC) codes

  • General

Cite this

Liu, P., Tarlé, S. A., Hajra, A., Claxton, D., Marlton, P., Freedman, M., ... Collins, F. S. (1993). Fusion between transcription factor CBFβ/PEBP2β and a myosin heavy chain in acute myeloid leukemia. Science, 261(5124), 1041-1044. https://doi.org/10.1126/science.8351518
Liu, Pu ; Tarlé, Susan A. ; Hajra, Amitav ; Claxton, David ; Marlton, Paula ; Freedman, Matthew ; Siciliano, Michael J. ; Collins, Francis S. / Fusion between transcription factor CBFβ/PEBP2β and a myosin heavy chain in acute myeloid leukemia. In: Science. 1993 ; Vol. 261, No. 5124. pp. 1041-1044.
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abstract = "The pericentric inversion of chromosome 16 [inv(16)(p13q22)] is a characteristic karyotypic abnormality associated with acute myeloid leukemia, most commonly of the M4Eo subtype. The 16p and 16q breakpoints were pinpointed by yeast artificial chromosome and cosmid cloning, and the two genes involved in this inversion were identified. On 16q the inversion occurred near the end of the coding region for CBFβ, also known as PEBP2β, a subunit of a heterodimeric transcription factor regulating genes expressed in T cells; on 16p a smooth muscle myosin heavy chain (SMMHC) gene (MYH11) was interrupted. In six of six inv(16) patient samples tested, an in-frame fusion messenger RNA was demonstrated that connected the first 165 amino acids of CBFβ with the tail region of SMMHC. The repeated coiled coil of SMMHC may result in dimerization of the CBFβ fusion protein, which in turn would lead to alterations in transcriptional regulation and contribute to leukemic transformation.",
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Liu, P, Tarlé, SA, Hajra, A, Claxton, D, Marlton, P, Freedman, M, Siciliano, MJ & Collins, FS 1993, 'Fusion between transcription factor CBFβ/PEBP2β and a myosin heavy chain in acute myeloid leukemia', Science, vol. 261, no. 5124, pp. 1041-1044. https://doi.org/10.1126/science.8351518

Fusion between transcription factor CBFβ/PEBP2β and a myosin heavy chain in acute myeloid leukemia. / Liu, Pu; Tarlé, Susan A.; Hajra, Amitav; Claxton, David; Marlton, Paula; Freedman, Matthew; Siciliano, Michael J.; Collins, Francis S.

In: Science, Vol. 261, No. 5124, 01.01.1993, p. 1041-1044.

Research output: Contribution to journalArticle

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AU - Liu, Pu

AU - Tarlé, Susan A.

AU - Hajra, Amitav

AU - Claxton, David

AU - Marlton, Paula

AU - Freedman, Matthew

AU - Siciliano, Michael J.

AU - Collins, Francis S.

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