Fvb/n mice: An inbred strain sensitive to the chemical induction of squamous cell carcinomas in the skin

Henry Hennings, Adam B. Glick, David T. Lowry, Ljubicka S. Krsmanovic, Linda M. Sly, Stuart H. Yuspa

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Abstract

The widespread use of FVB/N mice for the establishment of transgenic lines containing active oncogenes suggested the importance of testing the parent FVB/N mice for sensitivity to experimental carcinogenesis. After initiation of mouse skin by a single treatment with 7, 12-dimethylbenz[a]anthracene (DMBA) and promotion by 20 weekly applications of 12-O-tetradecanoylphorbol-13-acetate (TPA), the skin tumor incidence was compared in FVB/N mice, TPA-sensitive (SENCAR and CD-I) and TPA-resistant mice (BALB/c and C57BL/6). Initiation by 25 μg DMBA followed by promotion with a low dose of TPA (2 μg/week) induced one or more papillomas in only 25% of FVB/N mice, compared with 100% in SENCAR, 53% in CD-I, 17% in BALB/c and 0% in C57BL/6 mice. At a more effective dose of TPA (5 /ig/week), FVB/N mice initiated by 5, 25 or 100μg DMBA developed 3.4, 6.9 and 11.8 papillomas per mouse. In contrast, the incidence of squamous cell carcinomas (SCCs) (17-18/30 mice) did not increase with DMBA dose. TPA promotion of non-initiated mice induced only six papillomas, but three progressed to SCCs, a high rate of malignant conversion. Skin tumor induction by 20 weekly treatments with 10 μg DMBA produced few papillomas, but 50.0% of the papillomas progressed to carcinomas in FVB/N mice, compared with 9.15% in SENCAR, 37.5% in CD-I, 23.1% in BALB/c and 15.0% in C57CL/6 mice. The first carcinomas appeared after 14 weeks in FVB/N, 24 weeks in SENCAR, 26 weeks in CD-I and C57BL/6 and 34 weeks in BALB/c mice. Thus, FVB/N mice develop an unusually high incidence of SCCs after treatment with repeated DMBA, DMBA initiation-TPA promotion and even TPA alone.

Original languageEnglish (US)
Pages (from-to)2353-2358
Number of pages6
JournalCarcinogenesis
Volume14
Issue number11
DOIs
StatePublished - Nov 1 1993

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All Science Journal Classification (ASJC) codes

  • Cancer Research

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