FvSO regulates vegetative hyphal fusion, asexual growth, fumonisin B1 production, and virulence in Fusarium verticillioides

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Hyphal anastomosis is a hallmark of filamentous fungi and plays vital roles including cellular homoeostasis, interhyphal communication and nutrient translocation. Here we identify a gene, FvSO, in Fusarium verticillioides, a filamentous ascomycete causing maize ear and stalk rot and producing fumonisin mycotoxins. FvSO, like its Neurospora crassa homologue SO, is required for vegetative hyphal fusion. It is also essential for normal vegetative growth, sporulation, and pathogenesis. FvSO encodes a predicted WW domain protein and shares 70 % protein sequence identity with N. crassa SO. FvSO deletion mutants (Δ FvSO) had abnormal distribution of conidia size, and conidia of Δ FvSO germinated much later and slower than wild type. Δ FvSO was deficient in hyphal anastomosis, had slower radial growth and produced less fungal biomass than wild type. Δ FvSO were unable to perform anastomosis, a key feature of filamentous fungi. Interestingly, production of fumonisin B1 by Δ FvSO was significantly reduced compared to wild type. Additionally, Δ FvSO was nonpathogenic to corn ears, stalks and seedlings, likely due to defective growth and development. In conclusion, FvSO is essential for vegetative hyphal fusion and is required for normal vegetative growth and sporulation, normal levels of fumonisin production and pathogenicity in F. verticillioides. The pleiotropic nature of Δ FvSO phenotypes suggests that FvSO is likely involved in certain signalling pathways that regulate multiple cellular functions.

Original languageEnglish (US)
Pages (from-to)1158-1169
Number of pages12
JournalFungal Biology
Volume119
Issue number12
DOIs
StatePublished - Dec 1 2015

Fingerprint

corn ears
Neurospora crassa
fumonisin B1
fumonisins
Fusarium
virulence
sporulation
conidia
Fumonisins
vegetative growth
Virulence
Fungal Spores
fungi
Zea mays
Ear
corn stover
Fungi
Growth
maize
Ascomycota

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Infectious Diseases

Cite this

@article{25b0a04a59df412fb0bc6e5012f6f79e,
title = "FvSO regulates vegetative hyphal fusion, asexual growth, fumonisin B1 production, and virulence in Fusarium verticillioides",
abstract = "Hyphal anastomosis is a hallmark of filamentous fungi and plays vital roles including cellular homoeostasis, interhyphal communication and nutrient translocation. Here we identify a gene, FvSO, in Fusarium verticillioides, a filamentous ascomycete causing maize ear and stalk rot and producing fumonisin mycotoxins. FvSO, like its Neurospora crassa homologue SO, is required for vegetative hyphal fusion. It is also essential for normal vegetative growth, sporulation, and pathogenesis. FvSO encodes a predicted WW domain protein and shares 70 {\%} protein sequence identity with N. crassa SO. FvSO deletion mutants (Δ FvSO) had abnormal distribution of conidia size, and conidia of Δ FvSO germinated much later and slower than wild type. Δ FvSO was deficient in hyphal anastomosis, had slower radial growth and produced less fungal biomass than wild type. Δ FvSO were unable to perform anastomosis, a key feature of filamentous fungi. Interestingly, production of fumonisin B1 by Δ FvSO was significantly reduced compared to wild type. Additionally, Δ FvSO was nonpathogenic to corn ears, stalks and seedlings, likely due to defective growth and development. In conclusion, FvSO is essential for vegetative hyphal fusion and is required for normal vegetative growth and sporulation, normal levels of fumonisin production and pathogenicity in F. verticillioides. The pleiotropic nature of Δ FvSO phenotypes suggests that FvSO is likely involved in certain signalling pathways that regulate multiple cellular functions.",
author = "Li Guo and Wenner, {Nancy G.} and Kuldau, {Gretchen Anna}",
year = "2015",
month = "12",
day = "1",
doi = "10.1016/j.funbio.2015.08.013",
language = "English (US)",
volume = "119",
pages = "1158--1169",
journal = "Fungal Biology",
issn = "1878-6146",
publisher = "Elsevier",
number = "12",

}

TY - JOUR

T1 - FvSO regulates vegetative hyphal fusion, asexual growth, fumonisin B1 production, and virulence in Fusarium verticillioides

AU - Guo, Li

AU - Wenner, Nancy G.

AU - Kuldau, Gretchen Anna

PY - 2015/12/1

Y1 - 2015/12/1

N2 - Hyphal anastomosis is a hallmark of filamentous fungi and plays vital roles including cellular homoeostasis, interhyphal communication and nutrient translocation. Here we identify a gene, FvSO, in Fusarium verticillioides, a filamentous ascomycete causing maize ear and stalk rot and producing fumonisin mycotoxins. FvSO, like its Neurospora crassa homologue SO, is required for vegetative hyphal fusion. It is also essential for normal vegetative growth, sporulation, and pathogenesis. FvSO encodes a predicted WW domain protein and shares 70 % protein sequence identity with N. crassa SO. FvSO deletion mutants (Δ FvSO) had abnormal distribution of conidia size, and conidia of Δ FvSO germinated much later and slower than wild type. Δ FvSO was deficient in hyphal anastomosis, had slower radial growth and produced less fungal biomass than wild type. Δ FvSO were unable to perform anastomosis, a key feature of filamentous fungi. Interestingly, production of fumonisin B1 by Δ FvSO was significantly reduced compared to wild type. Additionally, Δ FvSO was nonpathogenic to corn ears, stalks and seedlings, likely due to defective growth and development. In conclusion, FvSO is essential for vegetative hyphal fusion and is required for normal vegetative growth and sporulation, normal levels of fumonisin production and pathogenicity in F. verticillioides. The pleiotropic nature of Δ FvSO phenotypes suggests that FvSO is likely involved in certain signalling pathways that regulate multiple cellular functions.

AB - Hyphal anastomosis is a hallmark of filamentous fungi and plays vital roles including cellular homoeostasis, interhyphal communication and nutrient translocation. Here we identify a gene, FvSO, in Fusarium verticillioides, a filamentous ascomycete causing maize ear and stalk rot and producing fumonisin mycotoxins. FvSO, like its Neurospora crassa homologue SO, is required for vegetative hyphal fusion. It is also essential for normal vegetative growth, sporulation, and pathogenesis. FvSO encodes a predicted WW domain protein and shares 70 % protein sequence identity with N. crassa SO. FvSO deletion mutants (Δ FvSO) had abnormal distribution of conidia size, and conidia of Δ FvSO germinated much later and slower than wild type. Δ FvSO was deficient in hyphal anastomosis, had slower radial growth and produced less fungal biomass than wild type. Δ FvSO were unable to perform anastomosis, a key feature of filamentous fungi. Interestingly, production of fumonisin B1 by Δ FvSO was significantly reduced compared to wild type. Additionally, Δ FvSO was nonpathogenic to corn ears, stalks and seedlings, likely due to defective growth and development. In conclusion, FvSO is essential for vegetative hyphal fusion and is required for normal vegetative growth and sporulation, normal levels of fumonisin production and pathogenicity in F. verticillioides. The pleiotropic nature of Δ FvSO phenotypes suggests that FvSO is likely involved in certain signalling pathways that regulate multiple cellular functions.

UR - http://www.scopus.com/inward/record.url?scp=84948114712&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84948114712&partnerID=8YFLogxK

U2 - 10.1016/j.funbio.2015.08.013

DO - 10.1016/j.funbio.2015.08.013

M3 - Article

VL - 119

SP - 1158

EP - 1169

JO - Fungal Biology

JF - Fungal Biology

SN - 1878-6146

IS - 12

ER -