GABA-dependent firing of glutamate-evoked action potentials at AMPA/kainate receptors in developing hypothalamic neurons

Xiao Bing Gao, Gong Chen, Anthony N. Van Den Pol

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Although it plays a major inhibitory role in the adult mammalian CNS, γ-aminobutyric acid (GABA) may have an excitatory function in developing neurons. The present study focuses on the dependence of glutamate on GABA to generate action potentials in developing hypothalamic neurons. Under conditions where glutamate by itself could not evoke an action potential, GABA facilitated glutamate-mediated depolarization to fire action potentials. This facilitation had a broad time window during the decaying phase of the GABA-mediated depolarization in developing neurons in culture. The glutamate- mediated depolarization was shunted only during the peak of GABA-mediated depolarization, but was facilitated after that. Similar results were obtained in the presence of 2-amino-5-phosphonopentanoic acid (AP5), indicating that GABA can facilitate glutamate responses independent of relief of the Mg2+ block of the N-methyl-D-aspartate (NMDA) receptor. This novel interaction between GABA- and glutamate-mediated excitation could play a role in strengthening neuronal circuits during early development and would exert a maximal effect if GABA and glutamate receptors were activated after a slight temporal delay.

Original languageEnglish (US)
Pages (from-to)716-726
Number of pages11
JournalJournal of neurophysiology
Volume79
Issue number2
DOIs
StatePublished - Feb 1998

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Kainic Acid Receptors
AMPA Receptors
Evoked Potentials
gamma-Aminobutyric Acid
Action Potentials
Glutamic Acid
Neurons
2-Amino-5-phosphonovalerate
Aminobutyrates
GABA Receptors
Glutamate Receptors
N-Methyl-D-Aspartate Receptors

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Physiology

Cite this

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GABA-dependent firing of glutamate-evoked action potentials at AMPA/kainate receptors in developing hypothalamic neurons. / Gao, Xiao Bing; Chen, Gong; Van Den Pol, Anthony N.

In: Journal of neurophysiology, Vol. 79, No. 2, 02.1998, p. 716-726.

Research output: Contribution to journalArticle

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