GAG-augmented polysaccharide hydrogel: A novel biocompatible and biodegradable material to support chondrogenesis

V. Franklin Sechriest, Yi Jian Miao, Christopher Niyibizi, Andrea Westerhausen-Larson, Howard W. Matthew, Christopher H. Evans, Freddie H. Fu, Jun Kyo Suh

Research output: Contribution to journalArticle

235 Citations (Scopus)

Abstract

The quality of articular cartilage engineered using a cell-polymer construct depends, in part, on the chemical composition of the biomaterial and whether that biomaterial can support the chondrocytic phenotype. Acknowledging the supportive influence of tissue-specific matrix molecules on the chondrocytic phenotype, we have combined chondroitin sulfate-A (CSA) and chitosan, a glycosaminoglycan (GAG) analog, to develop a novel biomaterial to support chondrogenesis. Chitosan is a polycationic repeating monosaccharide of β-1,4-linked glucosamine monomers with randomly located N-acetyl glucosamine units. Chitosan may be combined with the polyanionic CSA such that ionic crosslinking results in hydrogel formation. Bovine primary articular chondrocytes, when seeded onto a thin layer of CSA-chitosan, form discrete, focal adhesions to the material and maintain many characteristics of the differentiated chondrocytic phenotype, including round morphology, limited mitosis, collagen type II, and proteoglycan production. Our findings suggest CSA-chitosan may be well suited as a carrier material for the transplant of autologous chondrocytes or as a scaffold for the tissue engineering of cartilage-like tissue.

Original languageEnglish (US)
Pages (from-to)534-541
Number of pages8
JournalJournal of Biomedical Materials Research
Volume49
Issue number4
DOIs
StatePublished - Mar 15 2000

Fingerprint

Hydrogel
Chitosan
Biocompatible Materials
Polysaccharides
Glycosaminoglycans
Hydrogels
Chondroitin Sulfates
Biomaterials
Glucosamine
Cartilage
Tissue
Transplants
Collagen Type II
Monosaccharides
Autografts
Proteoglycans
Scaffolds (biology)
Tissue engineering
Collagen
Crosslinking

All Science Journal Classification (ASJC) codes

  • Biomaterials
  • Biomedical Engineering

Cite this

Sechriest, V. Franklin ; Miao, Yi Jian ; Niyibizi, Christopher ; Westerhausen-Larson, Andrea ; Matthew, Howard W. ; Evans, Christopher H. ; Fu, Freddie H. ; Suh, Jun Kyo. / GAG-augmented polysaccharide hydrogel : A novel biocompatible and biodegradable material to support chondrogenesis. In: Journal of Biomedical Materials Research. 2000 ; Vol. 49, No. 4. pp. 534-541.
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GAG-augmented polysaccharide hydrogel : A novel biocompatible and biodegradable material to support chondrogenesis. / Sechriest, V. Franklin; Miao, Yi Jian; Niyibizi, Christopher; Westerhausen-Larson, Andrea; Matthew, Howard W.; Evans, Christopher H.; Fu, Freddie H.; Suh, Jun Kyo.

In: Journal of Biomedical Materials Research, Vol. 49, No. 4, 15.03.2000, p. 534-541.

Research output: Contribution to journalArticle

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