Gallium induces the production of virulence factors in Pseudomonas aeruginosa

Rodolfo García-Contreras, Berenice Pérez-Eretza, Elizabeth Lira-Silva, Ricardo Jasso-Chávez, Rafael Coria-Jiménez, Adrián Rangel-Vega, Toshinari Maeda, Thomas K. Wood

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The novel antimicrobial gallium is a nonredox iron III analogue with bacteriostatic and bactericidal properties, effective for the treatment of Pseudomonas aeruginosa in vitro and in vivo in mouse and rabbit infection models. It interferes with iron metabolism, transport, and presumably its homeostasis. As gallium exerts its antimicrobial effects by competing with iron, we hypothesized that it ultimately will lead cells to an iron deficiency status. As iron deficiency promotes the expression of virulence factors in vitro and promotes the pathogenicity of P. aeruginosa in animal models, it is anticipated that treatment with gallium will also promote the production of virulence factors. To test this hypothesis, the reference strain PA14 and two clinical isolates from patients with cystic fibrosis were exposed to gallium, and their production of pyocyanin, rhamnolipids, elastase, alkaline protease, alginate, pyoverdine, and biofilm was determined. Gallium treatment induced the production of all the virulence factors tested in the three strains except for pyoverdine. In addition, as the Ga-induced virulence factors are quorum sensing controlled, co-administration of Ga and the quorum quencher brominated furanone C-30 was assayed, and it was found that C-30 alleviated growth inhibition from gallium. Hence, adding both C-30 and gallium may be more effective in the treatment of P. aeruginosa infections.

Original languageEnglish (US)
Pages (from-to)95-98
Number of pages4
JournalPathogens and disease
Volume70
Issue number1
DOIs
StatePublished - Feb 2014

Fingerprint

Gallium
Virulence Factors
Pseudomonas aeruginosa
Iron
Pyocyanine
Pseudomonas Infections
Quorum Sensing
Pancreatic Elastase
Therapeutics
Biofilms
Cystic Fibrosis
Virulence
Homeostasis
Animal Models
Rabbits
Growth
Infection

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology and Microbiology(all)
  • Microbiology (medical)
  • Infectious Diseases

Cite this

García-Contreras, R., Pérez-Eretza, B., Lira-Silva, E., Jasso-Chávez, R., Coria-Jiménez, R., Rangel-Vega, A., ... Wood, T. K. (2014). Gallium induces the production of virulence factors in Pseudomonas aeruginosa. Pathogens and disease, 70(1), 95-98. https://doi.org/10.1111/2049-632X.12105
García-Contreras, Rodolfo ; Pérez-Eretza, Berenice ; Lira-Silva, Elizabeth ; Jasso-Chávez, Ricardo ; Coria-Jiménez, Rafael ; Rangel-Vega, Adrián ; Maeda, Toshinari ; Wood, Thomas K. / Gallium induces the production of virulence factors in Pseudomonas aeruginosa. In: Pathogens and disease. 2014 ; Vol. 70, No. 1. pp. 95-98.
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García-Contreras, R, Pérez-Eretza, B, Lira-Silva, E, Jasso-Chávez, R, Coria-Jiménez, R, Rangel-Vega, A, Maeda, T & Wood, TK 2014, 'Gallium induces the production of virulence factors in Pseudomonas aeruginosa', Pathogens and disease, vol. 70, no. 1, pp. 95-98. https://doi.org/10.1111/2049-632X.12105

Gallium induces the production of virulence factors in Pseudomonas aeruginosa. / García-Contreras, Rodolfo; Pérez-Eretza, Berenice; Lira-Silva, Elizabeth; Jasso-Chávez, Ricardo; Coria-Jiménez, Rafael; Rangel-Vega, Adrián; Maeda, Toshinari; Wood, Thomas K.

In: Pathogens and disease, Vol. 70, No. 1, 02.2014, p. 95-98.

Research output: Contribution to journalArticle

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T1 - Gallium induces the production of virulence factors in Pseudomonas aeruginosa

AU - García-Contreras, Rodolfo

AU - Pérez-Eretza, Berenice

AU - Lira-Silva, Elizabeth

AU - Jasso-Chávez, Ricardo

AU - Coria-Jiménez, Rafael

AU - Rangel-Vega, Adrián

AU - Maeda, Toshinari

AU - Wood, Thomas K.

PY - 2014/2

Y1 - 2014/2

N2 - The novel antimicrobial gallium is a nonredox iron III analogue with bacteriostatic and bactericidal properties, effective for the treatment of Pseudomonas aeruginosa in vitro and in vivo in mouse and rabbit infection models. It interferes with iron metabolism, transport, and presumably its homeostasis. As gallium exerts its antimicrobial effects by competing with iron, we hypothesized that it ultimately will lead cells to an iron deficiency status. As iron deficiency promotes the expression of virulence factors in vitro and promotes the pathogenicity of P. aeruginosa in animal models, it is anticipated that treatment with gallium will also promote the production of virulence factors. To test this hypothesis, the reference strain PA14 and two clinical isolates from patients with cystic fibrosis were exposed to gallium, and their production of pyocyanin, rhamnolipids, elastase, alkaline protease, alginate, pyoverdine, and biofilm was determined. Gallium treatment induced the production of all the virulence factors tested in the three strains except for pyoverdine. In addition, as the Ga-induced virulence factors are quorum sensing controlled, co-administration of Ga and the quorum quencher brominated furanone C-30 was assayed, and it was found that C-30 alleviated growth inhibition from gallium. Hence, adding both C-30 and gallium may be more effective in the treatment of P. aeruginosa infections.

AB - The novel antimicrobial gallium is a nonredox iron III analogue with bacteriostatic and bactericidal properties, effective for the treatment of Pseudomonas aeruginosa in vitro and in vivo in mouse and rabbit infection models. It interferes with iron metabolism, transport, and presumably its homeostasis. As gallium exerts its antimicrobial effects by competing with iron, we hypothesized that it ultimately will lead cells to an iron deficiency status. As iron deficiency promotes the expression of virulence factors in vitro and promotes the pathogenicity of P. aeruginosa in animal models, it is anticipated that treatment with gallium will also promote the production of virulence factors. To test this hypothesis, the reference strain PA14 and two clinical isolates from patients with cystic fibrosis were exposed to gallium, and their production of pyocyanin, rhamnolipids, elastase, alkaline protease, alginate, pyoverdine, and biofilm was determined. Gallium treatment induced the production of all the virulence factors tested in the three strains except for pyoverdine. In addition, as the Ga-induced virulence factors are quorum sensing controlled, co-administration of Ga and the quorum quencher brominated furanone C-30 was assayed, and it was found that C-30 alleviated growth inhibition from gallium. Hence, adding both C-30 and gallium may be more effective in the treatment of P. aeruginosa infections.

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García-Contreras R, Pérez-Eretza B, Lira-Silva E, Jasso-Chávez R, Coria-Jiménez R, Rangel-Vega A et al. Gallium induces the production of virulence factors in Pseudomonas aeruginosa. Pathogens and disease. 2014 Feb;70(1):95-98. https://doi.org/10.1111/2049-632X.12105