Ganglioside GD3 enhances adherence of botulinum and tetanus neurotoxins to bovine brain synapsin I

Cara-Lynne Schengrund, Bibhuti R. DasGupta, Nancy J. Ringler

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Tetanus toxin (TTx) and botulinum toxin serotype A (BTxA), preincubated with trisialoganglioside GT1b, adhere to proteins present on blots of bovine synaptosomal proteins. Differential solubilization and ammonium sulfate fractionation provided material enriched in two proteins that appeared to be adhered to most strongly by the labeled neurotoxins. After excision of the appropriate bands from blots of electrophoretically separated proteins, N-terminal amino acid sequence analysis permitted identification of the proteins as synapsins Ia and Ib. Comparison of the effectiveness of different gangliosides at enhancing adherence of the neurotoxins to blots of synapsins Ia and Ib indicated that GD3 was most effective.

Original languageEnglish (US)
Pages (from-to)159-162
Number of pages4
JournalNeuroscience Letters
Volume158
Issue number2
DOIs
StatePublished - Aug 20 1993

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Synapsins
Brain
Neurotoxins
Proteins
Tetanus Toxin
Type A Botulinum Toxins
Gangliosides
Protein Sequence Analysis
Ammonium Sulfate
tetanospasmin
GD3 ganglioside

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

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abstract = "Tetanus toxin (TTx) and botulinum toxin serotype A (BTxA), preincubated with trisialoganglioside GT1b, adhere to proteins present on blots of bovine synaptosomal proteins. Differential solubilization and ammonium sulfate fractionation provided material enriched in two proteins that appeared to be adhered to most strongly by the labeled neurotoxins. After excision of the appropriate bands from blots of electrophoretically separated proteins, N-terminal amino acid sequence analysis permitted identification of the proteins as synapsins Ia and Ib. Comparison of the effectiveness of different gangliosides at enhancing adherence of the neurotoxins to blots of synapsins Ia and Ib indicated that GD3 was most effective.",
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Ganglioside GD3 enhances adherence of botulinum and tetanus neurotoxins to bovine brain synapsin I. / Schengrund, Cara-Lynne; DasGupta, Bibhuti R.; Ringler, Nancy J.

In: Neuroscience Letters, Vol. 158, No. 2, 20.08.1993, p. 159-162.

Research output: Contribution to journalArticle

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T1 - Ganglioside GD3 enhances adherence of botulinum and tetanus neurotoxins to bovine brain synapsin I

AU - Schengrund, Cara-Lynne

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