Gas-liquid chromatographic determination of T-2 toxin in plasma.

S. P. Swanson, V. Ramaswamy, V. R. Beasley, W. B. Buck, H. H. Burmeister

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

A gas-liquid chromatographic method for the determination of T-2 toxin in plasma is described. The toxin is extracted with benzene, washed with aqueous sodium hydroxide, and chromatographed on a small Florisil column; the heptafluorobutyryl derivative is prepared by reaction with heptafluorobutyrylimidazole. The T-2 HFB derivative is chromatographed on OV-1 at 230 degrees C and measured with an electron capture detector. Iso-T-2, an isomer of T-2 toxin, is added to samples as an internal standard before extraction. Recoveries averaged 98.0 +/- 5.5% at levels ranging from 50 to 1000 ng/mL. The limit of detection is 25 ng/mL.

Original languageEnglish (US)
Pages (from-to)909-912
Number of pages4
JournalJournal - Association of Official Analytical Chemists
Volume66
Issue number4
DOIs
StatePublished - Jul 1983

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

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