Gene expression in the ventral tegmental area of 5 pairs of rat lines selectively bred for high or low ethanol consumption

William J. McBride, Mark W. Kimpel, Jeanette N. McClintick, Zheng Ming Ding, Petri Hyytia, Giancarlo Colombo, Howard J. Edenberg, Lawrence Lumeng, Richard L. Bell

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The objective of this study was to determine if there are common innate differences in gene expression or gene pathways in the ventral tegmental area (VTA) among 5 different pairs of rat lines selectively bred for high (HEC) or low (LEC) ethanol consumption: (a) alcohol-preferring (P) vs. alcohol-non-preferring (NP) rats; (b) high-alcohol-drinking (HAD) vs. low-alcohol-drinking (LAD) rats (replicate line pairs 1 and 2); (c) ALKO alcohol (AA) vs. nonalcohol (ANA) rats; and (d) Sardinian alcohol-preferring (sP) vs. alcohol-nonpreferring (sNP) rats. Microarray analysis revealed between 370 and 1340 unique named genes that significantly differed in expression between the individual line-pairs. Analysis using Gene Ontology (GO) and Ingenuity Pathways information indicated significant categories and networks in common for up to 3 line-pairs, but not for all 5 line-pairs; moreover, there were few genes in common in these categories and networks. ANOVA of the combined data for the 5 line-pairs indicated 1295 significant (p < 0.01) differences in expression of named genes. Although no individual named gene was significant across all 5 line-pairs, there were 22 genes that overlapped in the same direction in 3 or 4 of the line-pairs. Overall, the findings suggest that (a) some biological categories or networks may be in common for subsets of line-pairs; and (b) regulation of different genes and/or combinations of multiple biological systems (e.g., transcription, synaptic function, intracellular signaling and protection against oxidative stress) within the VTA (possibly involving dopamine and glutamate) may be contributing to the disparate alcohol drinking behaviors of these line-pairs.

Original languageEnglish (US)
Pages (from-to)275-285
Number of pages11
JournalPharmacology Biochemistry and Behavior
Volume102
Issue number2
DOIs
StatePublished - Aug 1 2012

Fingerprint

Ventral Tegmental Area
Gene expression
Rats
Ethanol
Genes
Alcohols
Gene Expression
Alcohol Drinking
Drinking Behavior
Gene Ontology
Microarray Analysis
Oxidative stress
Glutamic Acid
Dopamine
Analysis of Variance
Oxidative Stress
Biological systems
Transcription
Microarrays
Analysis of variance (ANOVA)

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Toxicology
  • Pharmacology
  • Clinical Biochemistry
  • Biological Psychiatry
  • Behavioral Neuroscience

Cite this

McBride, William J. ; Kimpel, Mark W. ; McClintick, Jeanette N. ; Ding, Zheng Ming ; Hyytia, Petri ; Colombo, Giancarlo ; Edenberg, Howard J. ; Lumeng, Lawrence ; Bell, Richard L. / Gene expression in the ventral tegmental area of 5 pairs of rat lines selectively bred for high or low ethanol consumption. In: Pharmacology Biochemistry and Behavior. 2012 ; Vol. 102, No. 2. pp. 275-285.
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abstract = "The objective of this study was to determine if there are common innate differences in gene expression or gene pathways in the ventral tegmental area (VTA) among 5 different pairs of rat lines selectively bred for high (HEC) or low (LEC) ethanol consumption: (a) alcohol-preferring (P) vs. alcohol-non-preferring (NP) rats; (b) high-alcohol-drinking (HAD) vs. low-alcohol-drinking (LAD) rats (replicate line pairs 1 and 2); (c) ALKO alcohol (AA) vs. nonalcohol (ANA) rats; and (d) Sardinian alcohol-preferring (sP) vs. alcohol-nonpreferring (sNP) rats. Microarray analysis revealed between 370 and 1340 unique named genes that significantly differed in expression between the individual line-pairs. Analysis using Gene Ontology (GO) and Ingenuity Pathways information indicated significant categories and networks in common for up to 3 line-pairs, but not for all 5 line-pairs; moreover, there were few genes in common in these categories and networks. ANOVA of the combined data for the 5 line-pairs indicated 1295 significant (p < 0.01) differences in expression of named genes. Although no individual named gene was significant across all 5 line-pairs, there were 22 genes that overlapped in the same direction in 3 or 4 of the line-pairs. Overall, the findings suggest that (a) some biological categories or networks may be in common for subsets of line-pairs; and (b) regulation of different genes and/or combinations of multiple biological systems (e.g., transcription, synaptic function, intracellular signaling and protection against oxidative stress) within the VTA (possibly involving dopamine and glutamate) may be contributing to the disparate alcohol drinking behaviors of these line-pairs.",
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McBride, WJ, Kimpel, MW, McClintick, JN, Ding, ZM, Hyytia, P, Colombo, G, Edenberg, HJ, Lumeng, L & Bell, RL 2012, 'Gene expression in the ventral tegmental area of 5 pairs of rat lines selectively bred for high or low ethanol consumption', Pharmacology Biochemistry and Behavior, vol. 102, no. 2, pp. 275-285. https://doi.org/10.1016/j.pbb.2012.04.016

Gene expression in the ventral tegmental area of 5 pairs of rat lines selectively bred for high or low ethanol consumption. / McBride, William J.; Kimpel, Mark W.; McClintick, Jeanette N.; Ding, Zheng Ming; Hyytia, Petri; Colombo, Giancarlo; Edenberg, Howard J.; Lumeng, Lawrence; Bell, Richard L.

In: Pharmacology Biochemistry and Behavior, Vol. 102, No. 2, 01.08.2012, p. 275-285.

Research output: Contribution to journalArticle

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T1 - Gene expression in the ventral tegmental area of 5 pairs of rat lines selectively bred for high or low ethanol consumption

AU - McBride, William J.

AU - Kimpel, Mark W.

AU - McClintick, Jeanette N.

AU - Ding, Zheng Ming

AU - Hyytia, Petri

AU - Colombo, Giancarlo

AU - Edenberg, Howard J.

AU - Lumeng, Lawrence

AU - Bell, Richard L.

PY - 2012/8/1

Y1 - 2012/8/1

N2 - The objective of this study was to determine if there are common innate differences in gene expression or gene pathways in the ventral tegmental area (VTA) among 5 different pairs of rat lines selectively bred for high (HEC) or low (LEC) ethanol consumption: (a) alcohol-preferring (P) vs. alcohol-non-preferring (NP) rats; (b) high-alcohol-drinking (HAD) vs. low-alcohol-drinking (LAD) rats (replicate line pairs 1 and 2); (c) ALKO alcohol (AA) vs. nonalcohol (ANA) rats; and (d) Sardinian alcohol-preferring (sP) vs. alcohol-nonpreferring (sNP) rats. Microarray analysis revealed between 370 and 1340 unique named genes that significantly differed in expression between the individual line-pairs. Analysis using Gene Ontology (GO) and Ingenuity Pathways information indicated significant categories and networks in common for up to 3 line-pairs, but not for all 5 line-pairs; moreover, there were few genes in common in these categories and networks. ANOVA of the combined data for the 5 line-pairs indicated 1295 significant (p < 0.01) differences in expression of named genes. Although no individual named gene was significant across all 5 line-pairs, there were 22 genes that overlapped in the same direction in 3 or 4 of the line-pairs. Overall, the findings suggest that (a) some biological categories or networks may be in common for subsets of line-pairs; and (b) regulation of different genes and/or combinations of multiple biological systems (e.g., transcription, synaptic function, intracellular signaling and protection against oxidative stress) within the VTA (possibly involving dopamine and glutamate) may be contributing to the disparate alcohol drinking behaviors of these line-pairs.

AB - The objective of this study was to determine if there are common innate differences in gene expression or gene pathways in the ventral tegmental area (VTA) among 5 different pairs of rat lines selectively bred for high (HEC) or low (LEC) ethanol consumption: (a) alcohol-preferring (P) vs. alcohol-non-preferring (NP) rats; (b) high-alcohol-drinking (HAD) vs. low-alcohol-drinking (LAD) rats (replicate line pairs 1 and 2); (c) ALKO alcohol (AA) vs. nonalcohol (ANA) rats; and (d) Sardinian alcohol-preferring (sP) vs. alcohol-nonpreferring (sNP) rats. Microarray analysis revealed between 370 and 1340 unique named genes that significantly differed in expression between the individual line-pairs. Analysis using Gene Ontology (GO) and Ingenuity Pathways information indicated significant categories and networks in common for up to 3 line-pairs, but not for all 5 line-pairs; moreover, there were few genes in common in these categories and networks. ANOVA of the combined data for the 5 line-pairs indicated 1295 significant (p < 0.01) differences in expression of named genes. Although no individual named gene was significant across all 5 line-pairs, there were 22 genes that overlapped in the same direction in 3 or 4 of the line-pairs. Overall, the findings suggest that (a) some biological categories or networks may be in common for subsets of line-pairs; and (b) regulation of different genes and/or combinations of multiple biological systems (e.g., transcription, synaptic function, intracellular signaling and protection against oxidative stress) within the VTA (possibly involving dopamine and glutamate) may be contributing to the disparate alcohol drinking behaviors of these line-pairs.

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