Gene expression of transferrin and tranferrin receptor in brains of control vs. iron-deficient rats

Jian Han, Jonathan R. Day, James Connor, John L. Beard

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

The mechanism of the regulation of transferrin (Tf) and transferrin receptor (TfR) levels in rat brain by dietary iron status is not fully elucidated. We examined Tf and TfR protein and mRNA contents in various brain regions affected by dietary iron deficiency, and analyzed the relationships between protein and mRNA contents in brains of control vs. iron-deficient rats. In a region-specific fashion, iron-deficient diet decreased significantly brain iron concentration by 22-63%, and increased Tf level by 22-130% and TfR level by 74% in thalamus and 40% in cortex. Tf mRNA content decreased by 20-50% in most brain regions demonstrating inverse correlation of Tf and its mRNA in response to iron deficiency. TfR mRNA levels remained unaffected by iron status. The corpus callosum, white matter of the cerebellum and lateral ventricles expressed highest levels of Tf mRNA, whereas TfR mRNA levels was lowest in these regions, but highest in cortex, hippocampus, and the gray matter of the cerebellum. The data demonstrate that the cells in brain have the capacity to maintain minimum iron levels during iron deficiency. This capacity may be associated with increased iron-Tf uptake from plasma, stabilization of TfR mRNA, or increased Tf mRNA translation efficiency in specific cell types within the brain.

Original languageEnglish (US)
Pages (from-to)1-10
Number of pages10
JournalNutritional Neuroscience
Volume6
Issue number1
DOIs
StatePublished - Feb 1 2003

Fingerprint

Transferrin Receptors
transferrin
Transferrin
Iron
iron
brain
Gene Expression
gene expression
Messenger RNA
receptors
rats
Brain
Dietary Iron
Cerebellum
cerebellum
Corpus Callosum
Lateral Ventricles
Protein Biosynthesis
Thalamus
cortex

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Neuroscience(all)
  • Nutrition and Dietetics

Cite this

@article{572100fdfe7648c68262377843944089,
title = "Gene expression of transferrin and tranferrin receptor in brains of control vs. iron-deficient rats",
abstract = "The mechanism of the regulation of transferrin (Tf) and transferrin receptor (TfR) levels in rat brain by dietary iron status is not fully elucidated. We examined Tf and TfR protein and mRNA contents in various brain regions affected by dietary iron deficiency, and analyzed the relationships between protein and mRNA contents in brains of control vs. iron-deficient rats. In a region-specific fashion, iron-deficient diet decreased significantly brain iron concentration by 22-63{\%}, and increased Tf level by 22-130{\%} and TfR level by 74{\%} in thalamus and 40{\%} in cortex. Tf mRNA content decreased by 20-50{\%} in most brain regions demonstrating inverse correlation of Tf and its mRNA in response to iron deficiency. TfR mRNA levels remained unaffected by iron status. The corpus callosum, white matter of the cerebellum and lateral ventricles expressed highest levels of Tf mRNA, whereas TfR mRNA levels was lowest in these regions, but highest in cortex, hippocampus, and the gray matter of the cerebellum. The data demonstrate that the cells in brain have the capacity to maintain minimum iron levels during iron deficiency. This capacity may be associated with increased iron-Tf uptake from plasma, stabilization of TfR mRNA, or increased Tf mRNA translation efficiency in specific cell types within the brain.",
author = "Jian Han and Day, {Jonathan R.} and James Connor and Beard, {John L.}",
year = "2003",
month = "2",
day = "1",
doi = "10.1080/1028415021000042811",
language = "English (US)",
volume = "6",
pages = "1--10",
journal = "Nutritional Neuroscience",
issn = "1028-415X",
publisher = "Maney Publishing",
number = "1",

}

Gene expression of transferrin and tranferrin receptor in brains of control vs. iron-deficient rats. / Han, Jian; Day, Jonathan R.; Connor, James; Beard, John L.

In: Nutritional Neuroscience, Vol. 6, No. 1, 01.02.2003, p. 1-10.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Gene expression of transferrin and tranferrin receptor in brains of control vs. iron-deficient rats

AU - Han, Jian

AU - Day, Jonathan R.

AU - Connor, James

AU - Beard, John L.

PY - 2003/2/1

Y1 - 2003/2/1

N2 - The mechanism of the regulation of transferrin (Tf) and transferrin receptor (TfR) levels in rat brain by dietary iron status is not fully elucidated. We examined Tf and TfR protein and mRNA contents in various brain regions affected by dietary iron deficiency, and analyzed the relationships between protein and mRNA contents in brains of control vs. iron-deficient rats. In a region-specific fashion, iron-deficient diet decreased significantly brain iron concentration by 22-63%, and increased Tf level by 22-130% and TfR level by 74% in thalamus and 40% in cortex. Tf mRNA content decreased by 20-50% in most brain regions demonstrating inverse correlation of Tf and its mRNA in response to iron deficiency. TfR mRNA levels remained unaffected by iron status. The corpus callosum, white matter of the cerebellum and lateral ventricles expressed highest levels of Tf mRNA, whereas TfR mRNA levels was lowest in these regions, but highest in cortex, hippocampus, and the gray matter of the cerebellum. The data demonstrate that the cells in brain have the capacity to maintain minimum iron levels during iron deficiency. This capacity may be associated with increased iron-Tf uptake from plasma, stabilization of TfR mRNA, or increased Tf mRNA translation efficiency in specific cell types within the brain.

AB - The mechanism of the regulation of transferrin (Tf) and transferrin receptor (TfR) levels in rat brain by dietary iron status is not fully elucidated. We examined Tf and TfR protein and mRNA contents in various brain regions affected by dietary iron deficiency, and analyzed the relationships between protein and mRNA contents in brains of control vs. iron-deficient rats. In a region-specific fashion, iron-deficient diet decreased significantly brain iron concentration by 22-63%, and increased Tf level by 22-130% and TfR level by 74% in thalamus and 40% in cortex. Tf mRNA content decreased by 20-50% in most brain regions demonstrating inverse correlation of Tf and its mRNA in response to iron deficiency. TfR mRNA levels remained unaffected by iron status. The corpus callosum, white matter of the cerebellum and lateral ventricles expressed highest levels of Tf mRNA, whereas TfR mRNA levels was lowest in these regions, but highest in cortex, hippocampus, and the gray matter of the cerebellum. The data demonstrate that the cells in brain have the capacity to maintain minimum iron levels during iron deficiency. This capacity may be associated with increased iron-Tf uptake from plasma, stabilization of TfR mRNA, or increased Tf mRNA translation efficiency in specific cell types within the brain.

UR - http://www.scopus.com/inward/record.url?scp=0037309720&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037309720&partnerID=8YFLogxK

U2 - 10.1080/1028415021000042811

DO - 10.1080/1028415021000042811

M3 - Article

VL - 6

SP - 1

EP - 10

JO - Nutritional Neuroscience

JF - Nutritional Neuroscience

SN - 1028-415X

IS - 1

ER -