Gene expression profiles underlying alternative caste phenotypes in a highly eusocial bee, Melipona quadrifasciata

C. C. Judice, M. F. Carazzole, Fernanda Festa, M. C. Sogayar, K. Hartfelder, G. A.G. Pereira

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

To evaluate caste-biased gene expression in Melipona quadrifasciata, a stingless bee, we generated 1278 ESTs using Representational Difference Analysis. Most annotated sequences were similar to honey bee genes of unknown function. Only few queen-biased sequences had their putative function assigned by sequence comparison, contrasting with the worker-biased ESTs. The expression of six annotated genes connected to caste specificity was validated by real time PCR. Interestingly, queens that were developmentally induced by treatment with a juvenile hormone analogue displayed an expression profile clearly different from natural queens for this set of genes. In summary, this study represents an important first step in applying a comparative genomic approach to queen/worker polyphenism in the bee.

Original languageEnglish (US)
Pages (from-to)33-44
Number of pages12
JournalInsect Molecular Biology
Volume15
Issue number1
DOIs
StatePublished - Feb 1 2006

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Insect Science

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