Gene expression variation resolves species and individual strains among coral-associated dinoflagellates within the genus symbiodinium

John E. Parkinson, Sebastian Baumgarten, Craig T. Michell, Iliana B. Baums, Todd C. LaJeunesse, Christian R. Voolstra

Research output: Contribution to journalArticlepeer-review

56 Scopus citations

Abstract

Reef-building corals depend on symbiotic mutualisms with photosynthetic dinoflagellates in the genus Symbiodinium. This large microalgal group comprises many highly divergent lineages ("Clades A-I") and hundreds of undescribed species. Given their ecological importance, efforts have turned to genomic approaches to characterize the functional ecology of Symbiodinium. To date, investigators have only compared gene expression between representatives from separate clades-the equivalent of contrasting genera or families inother dinoflagellategroups-makingit impossible todistinguishbetweenclade-level andspecies-level functional differences. Here,we examined the transcriptomes of four specieswithin one Symbiodiniumclade (Clade B) at ~20,000 orthologous genes, as well as multiple isoclonal cell lines within species (i.e., cultured strains). These species span two major adaptive radiations within Clade B, each encompassing both host-specialized and ecologically cryptic taxa. Species-specific expression differences were consistently enriched for photosynthesis-related genes, likely reflecting selection pressures driving niche diversification. Transcriptional variation among strains involved fatty acid metabolism and biosynthesis pathways. Such differences among individuals are potentially amajor source of physiological variation, contributing to the functional diversity of coral holobionts composed of unique host-symbiont genotype pairings. Our findings expand the genomic resources available for this important symbiont group and emphasize the power of comparative transcriptomics as amethod for studying speciation processes and interindividual variation in nonmodel organisms.

Original languageEnglish (US)
Pages (from-to)665-680
Number of pages16
JournalGenome biology and evolution
Volume8
Issue number3
DOIs
StatePublished - Mar 2016

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Genetics

Fingerprint Dive into the research topics of 'Gene expression variation resolves species and individual strains among coral-associated dinoflagellates within the genus symbiodinium'. Together they form a unique fingerprint.

Cite this