Abstract
Bacterial wilt (BW) caused by Ralstonia solanacearum (Rs) is an important quarantine disease that spreads worldwide and infects hundreds of plant species. The BW defense response of potato is a complicated continuous process, which involves transcription of a battery of genes. The molecular mechanisms of potato-Rs interactions are poorly understood. In this study, we combined suppression subtractive hybridization and macroarray hybridization to identify genes that are differentially expressed during the incompatible interaction between Rs and potato. In total, 302 differentially expressed genes were identified and classified into 12 groups according to their putative biological functions. Of 302 genes, 81 were considered as Rs resistance-related genes based on the homology to genes of known function, and they have putative roles in pathogen recognition, signal transduction, transcription factor functioning, hypersensitive response, systemic acquired resistance, and cell rescue and protection. Additionally, 50 out of 302 genes had no match or low similarity in the NCBI databases, and they may represent novel genes. Of seven interesting genes analyzed via RNA gel blot and semi-quantitative RT-PCR, six were induced, one was suppressed, and all had different transcription patterns. The results demonstrate that the response of potato against Rs is rapid and involves the induction of numerous various genes. The genes identified in this study add to our knowledge of potato resistance to Rs.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 685-695 |
| Number of pages | 11 |
| Journal | Russian Journal of Plant Physiology |
| Volume | 57 |
| Issue number | 5 |
| DOIs | |
| State | Published - Sep 9 2010 |
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All Science Journal Classification (ASJC) codes
- Plant Science
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Gene Transcription Analysis during Interaction between Potato and Ralstonia solanacearum1. / Li, G. C.; Jin, L. P.; Wang, X. W.; Xie, K. Y.; Yang, Yinong; van der Vossen, E. A G; Huang, S. W.; Qu, D. Y.
In: Russian Journal of Plant Physiology, Vol. 57, No. 5, 09.09.2010, p. 685-695.Research output: Contribution to journal › Article
TY - JOUR
T1 - Gene Transcription Analysis during Interaction between Potato and Ralstonia solanacearum1
AU - Li, G. C.
AU - Jin, L. P.
AU - Wang, X. W.
AU - Xie, K. Y.
AU - Yang, Yinong
AU - van der Vossen, E. A G
AU - Huang, S. W.
AU - Qu, D. Y.
PY - 2010/9/9
Y1 - 2010/9/9
N2 - Bacterial wilt (BW) caused by Ralstonia solanacearum (Rs) is an important quarantine disease that spreads worldwide and infects hundreds of plant species. The BW defense response of potato is a complicated continuous process, which involves transcription of a battery of genes. The molecular mechanisms of potato-Rs interactions are poorly understood. In this study, we combined suppression subtractive hybridization and macroarray hybridization to identify genes that are differentially expressed during the incompatible interaction between Rs and potato. In total, 302 differentially expressed genes were identified and classified into 12 groups according to their putative biological functions. Of 302 genes, 81 were considered as Rs resistance-related genes based on the homology to genes of known function, and they have putative roles in pathogen recognition, signal transduction, transcription factor functioning, hypersensitive response, systemic acquired resistance, and cell rescue and protection. Additionally, 50 out of 302 genes had no match or low similarity in the NCBI databases, and they may represent novel genes. Of seven interesting genes analyzed via RNA gel blot and semi-quantitative RT-PCR, six were induced, one was suppressed, and all had different transcription patterns. The results demonstrate that the response of potato against Rs is rapid and involves the induction of numerous various genes. The genes identified in this study add to our knowledge of potato resistance to Rs.
AB - Bacterial wilt (BW) caused by Ralstonia solanacearum (Rs) is an important quarantine disease that spreads worldwide and infects hundreds of plant species. The BW defense response of potato is a complicated continuous process, which involves transcription of a battery of genes. The molecular mechanisms of potato-Rs interactions are poorly understood. In this study, we combined suppression subtractive hybridization and macroarray hybridization to identify genes that are differentially expressed during the incompatible interaction between Rs and potato. In total, 302 differentially expressed genes were identified and classified into 12 groups according to their putative biological functions. Of 302 genes, 81 were considered as Rs resistance-related genes based on the homology to genes of known function, and they have putative roles in pathogen recognition, signal transduction, transcription factor functioning, hypersensitive response, systemic acquired resistance, and cell rescue and protection. Additionally, 50 out of 302 genes had no match or low similarity in the NCBI databases, and they may represent novel genes. Of seven interesting genes analyzed via RNA gel blot and semi-quantitative RT-PCR, six were induced, one was suppressed, and all had different transcription patterns. The results demonstrate that the response of potato against Rs is rapid and involves the induction of numerous various genes. The genes identified in this study add to our knowledge of potato resistance to Rs.
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U2 - 10.1134/S1021443710050122
DO - 10.1134/S1021443710050122
M3 - Article
VL - 57
SP - 685
EP - 695
JO - Russian Journal of Plant Physiology
JF - Russian Journal of Plant Physiology
SN - 1021-4437
IS - 5
ER -