Gene Transcription Analysis during Interaction between Potato and Ralstonia solanacearum1

G. C. Li, L. P. Jin, X. W. Wang, K. Y. Xie, Yinong Yang, E. A G van der Vossen, S. W. Huang, D. Y. Qu

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Bacterial wilt (BW) caused by Ralstonia solanacearum (Rs) is an important quarantine disease that spreads worldwide and infects hundreds of plant species. The BW defense response of potato is a complicated continuous process, which involves transcription of a battery of genes. The molecular mechanisms of potato-Rs interactions are poorly understood. In this study, we combined suppression subtractive hybridization and macroarray hybridization to identify genes that are differentially expressed during the incompatible interaction between Rs and potato. In total, 302 differentially expressed genes were identified and classified into 12 groups according to their putative biological functions. Of 302 genes, 81 were considered as Rs resistance-related genes based on the homology to genes of known function, and they have putative roles in pathogen recognition, signal transduction, transcription factor functioning, hypersensitive response, systemic acquired resistance, and cell rescue and protection. Additionally, 50 out of 302 genes had no match or low similarity in the NCBI databases, and they may represent novel genes. Of seven interesting genes analyzed via RNA gel blot and semi-quantitative RT-PCR, six were induced, one was suppressed, and all had different transcription patterns. The results demonstrate that the response of potato against Rs is rapid and involves the induction of numerous various genes. The genes identified in this study add to our knowledge of potato resistance to Rs.

Original languageEnglish (US)
Pages (from-to)685-695
Number of pages11
JournalRussian Journal of Plant Physiology
Volume57
Issue number5
DOIs
StatePublished - Sep 9 2010

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Ralstonia
transcription (genetics)
potatoes
Ralstonia solanacearum
genes
bacterial wilt
systemic acquired resistance
suppression subtractive hybridization
hypersensitive response
quarantine
signal transduction

All Science Journal Classification (ASJC) codes

  • Plant Science

Cite this

Li, G. C. ; Jin, L. P. ; Wang, X. W. ; Xie, K. Y. ; Yang, Yinong ; van der Vossen, E. A G ; Huang, S. W. ; Qu, D. Y. / Gene Transcription Analysis during Interaction between Potato and Ralstonia solanacearum1. In: Russian Journal of Plant Physiology. 2010 ; Vol. 57, No. 5. pp. 685-695.
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Li, GC, Jin, LP, Wang, XW, Xie, KY, Yang, Y, van der Vossen, EAG, Huang, SW & Qu, DY 2010, 'Gene Transcription Analysis during Interaction between Potato and Ralstonia solanacearum1', Russian Journal of Plant Physiology, vol. 57, no. 5, pp. 685-695. https://doi.org/10.1134/S1021443710050122

Gene Transcription Analysis during Interaction between Potato and Ralstonia solanacearum1. / Li, G. C.; Jin, L. P.; Wang, X. W.; Xie, K. Y.; Yang, Yinong; van der Vossen, E. A G; Huang, S. W.; Qu, D. Y.

In: Russian Journal of Plant Physiology, Vol. 57, No. 5, 09.09.2010, p. 685-695.

Research output: Contribution to journalArticle

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