Generation and infection of organotypic cultures with Epstein-Barr virus

Rachel M. Temple, Craig Meyers, Clare E. Sample

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

While numerous model systems are available to study EBV latency in B cells and have contributed greatly to our understanding of the role of these cells in the viral life cycle, models to study the EBV life cycle in epithelial cells in vitro are lacking. Epithelial cells are poorly infected in vitro, and EBV-infected cell lines have not been successfully obtained from epithelial tumors. Recently, we have demonstrated that organotypic cultures of oral keratinocytes can be used as a model to study EBV infection in the epithelial tissue. These “raft” cultures generate a stratified tissue resembling the epithelium seen in vivo with a proliferating basal layer and differentiating suprabasal layers. Here, we describe generation of EBV-infected raft cultures established from primary oral mucosal epithelial cells, which exhibit high levels of productive replication induced by differentiation, as well as methods to analyze EBV infection.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages65-78
Number of pages14
DOIs
StatePublished - Jan 1 2017

Publication series

NameMethods in Molecular Biology
Volume1532
ISSN (Print)1064-3745

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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    Temple, R. M., Meyers, C., & Sample, C. E. (2017). Generation and infection of organotypic cultures with Epstein-Barr virus. In Methods in Molecular Biology (pp. 65-78). (Methods in Molecular Biology; Vol. 1532). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6655-4_4