Generation of a large combinatorial library of the immunoglobulin repertoire in phage lambda

William D. Huse, Lakshmi Sastry, Sheila A. Iverson, Angray S. Kang, Michelle Alting-Mees, Dennis R. Burton, Stephen Benkovic, Richard A. Lerner

Research output: Contribution to journalArticle

653 Scopus citations

Abstract

A novel bacteriophage lambda vector system was used to express in Escherichia coli a combinatorial library of Fab fragments of the mouse antibody repertoire. The system allows rapid and easy identification of monoclonal Fab fragments in a form suitable for genetic manipulation. It was possible to generate, in 2 weeks, large numbers of monoclonal Fab fragments against a transition state analog hapten. The methods described may supersede present-day hybridoma technology and facilitate the production of catalytic and other antibodies.

Original languageEnglish (US)
Pages (from-to)1275-1281
Number of pages7
JournalScience
Volume246
Issue number4935
DOIs
Publication statusPublished - Jan 1 1989

    Fingerprint

All Science Journal Classification (ASJC) codes

  • General

Cite this

Huse, W. D., Sastry, L., Iverson, S. A., Kang, A. S., Alting-Mees, M., Burton, D. R., ... Lerner, R. A. (1989). Generation of a large combinatorial library of the immunoglobulin repertoire in phage lambda. Science, 246(4935), 1275-1281. https://doi.org/10.1126/science.2531466