Generation of a large combinatorial library of the immunoglobulin repertoire in phage lambda

William D. Huse, Lakshmi Sastry, Sheila A. Iverson, Angray S. Kang, Michelle Alting-Mees, Dennis R. Burton, Stephen J. Benkovic, Richard A. Lerner

Research output: Contribution to journalArticlepeer-review

668 Scopus citations

Abstract

A novel bacteriophage lambda vector system was used to express in Escherichia coli a combinatorial library of Fab fragments of the mouse antibody repertoire. The system allows rapid and easy identification of monoclonal Fab fragments in a form suitable for genetic manipulation. It was possible to generate, in 2 weeks, large numbers of monoclonal Fab fragments against a transition state analog hapten. The methods described may supersede present-day hybridoma technology and facilitate the production of catalytic and other antibodies.

Original languageEnglish (US)
Pages (from-to)1275-1281
Number of pages7
JournalScience
Volume246
Issue number4935
DOIs
StatePublished - 1989

All Science Journal Classification (ASJC) codes

  • General

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