Generation of potentially bioactive ergosterol-derived products following pulsed ultraviolet light exposure of mushrooms (Agaricus bisporus)

Michael D. Kalaras, Robert B. Beelman, Michael F. Holick, Ryan Elias

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The production of vitamin D2 from ergosterol in mushrooms upon exposure to ultraviolet (UV) irradiation has been well established in recent years. However, the effect of this treatment on the generation of non-vitamin D2 products of ergosterol in mushrooms has not been reported. In this study, the ergosterol-derived photoproducts previtamin D2, lumisterol2 and tachysterol2 were, for the first time, identified and quantified in white button mushrooms (Agaricus bisporus) following treatment with pulsed UV (PUV) light. Mushrooms were treated with up to 60 pulses of PUV irradiation and the formation of major photoproducts was observed to increase as a function of dose. Vitamin D2 was the most abundant product, followed by previtamin D2, lumisterol2 and tachysterol2 in order of decreasing abundance. Untreated mushroom samples were not observed to contain detectable levels of any photoproduct. This study shows for the first time the production of these photoproducts in UV irradiated mushrooms. A complete understanding of the potential biological significance of these products remains to be seen.

Original languageEnglish (US)
Pages (from-to)396-401
Number of pages6
JournalFood Chemistry
Volume135
Issue number2
DOIs
StatePublished - Nov 15 2012

Fingerprint

Agaricus
Ergosterol
Agaricus bisporus
ergosterol
Agaricales
Ultraviolet Rays
mushrooms
Ergocalciferols
ultraviolet radiation
Irradiation
ergocalciferol
irradiation
dosage

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Food Science
  • Medicine(all)

Cite this

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title = "Generation of potentially bioactive ergosterol-derived products following pulsed ultraviolet light exposure of mushrooms (Agaricus bisporus)",
abstract = "The production of vitamin D2 from ergosterol in mushrooms upon exposure to ultraviolet (UV) irradiation has been well established in recent years. However, the effect of this treatment on the generation of non-vitamin D2 products of ergosterol in mushrooms has not been reported. In this study, the ergosterol-derived photoproducts previtamin D2, lumisterol2 and tachysterol2 were, for the first time, identified and quantified in white button mushrooms (Agaricus bisporus) following treatment with pulsed UV (PUV) light. Mushrooms were treated with up to 60 pulses of PUV irradiation and the formation of major photoproducts was observed to increase as a function of dose. Vitamin D2 was the most abundant product, followed by previtamin D2, lumisterol2 and tachysterol2 in order of decreasing abundance. Untreated mushroom samples were not observed to contain detectable levels of any photoproduct. This study shows for the first time the production of these photoproducts in UV irradiated mushrooms. A complete understanding of the potential biological significance of these products remains to be seen.",
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Generation of potentially bioactive ergosterol-derived products following pulsed ultraviolet light exposure of mushrooms (Agaricus bisporus). / Kalaras, Michael D.; Beelman, Robert B.; Holick, Michael F.; Elias, Ryan.

In: Food Chemistry, Vol. 135, No. 2, 15.11.2012, p. 396-401.

Research output: Contribution to journalArticle

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