Genes differentially regulated by NKX2-3 in B cells between ulcerative colitis and Crohn's disease patients and possible involvement of EGR1

Wei Yu, Zhenwu Lin, John P. Hegarty, Xi Chen, Ashley A. Kelly, Yunhua Wang, Lisa Poritz, Walter Koltun

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Abstract

Ulcerative colitis (UC) and Crohn's disease (CD) are two related yet different forms of chronic intestinal inflammation. We investigated the genes regulated by NKX2-3 in B cells from a UC patient by cDNA microarray and compared the results to those genes regulated by NKX2-3 in B cells from a CD patient. Genes regulated by NKX2-3 in B cells from UC were mainly involved in cell growth, inflammation, and immune response. Among the genes regulated by NKX2-3 in both UC and CD, expression of 145 genes was similarly altered and 34 genes was differentially affected by NKX2-3 knockdown. EGR1 was up-regulated in NKX2-3 knockdown B cells from UC while down-regulated in NKX2-3 knockdown B cells from CD. mRNA expressions of NKX2-3 and EGR1 were increased in diseased intestinal tissues from 19 CD patients. NKX2-3 may play different roles in UC and CD pathogenesis by differential regulation of EGR1.

Original languageEnglish (US)
Pages (from-to)889-899
Number of pages11
JournalInflammation
Volume35
Issue number3
DOIs
Publication statusPublished - Jun 1 2012

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All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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