Genes encoding the H,K-ATPase α and Na,K-ATPase α3 subunits are linked on mouse Chromosome 7 and human Chromosome 19

Danielle Malo, Philippe Gros, Anne Bergmann, Barbara Trask, Harvey W. Mohrenweiser, Victor A. Canfield, Robert Levenson

Research output: Contribution to journalArticle

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Abstract

We have used linkage analysis and fluorescence in situ hybridization to determine the chromosomal organization and location of the mouse (Atp4a) and human (ATP4A) genes encoding the H,K-ATPase α subunit. Linkage analysis in recombinant inbred (BXD) strains of mice localized Atp4a to mouse Chromosome (Chr) 7. Segregation of restriction fragment length polymorphisms in backcross progeny of Mus musculusxMus spretus mating confirmed this assignment and indicates that Atp4a and Atp1a3 (gene encoding the murine Na,K-ATPase α3 subunit) are linked and separated by a distance of ∼2 cM. Analysis of the segregation of simple sequence repeats suggested the gene order centromere-D7Mit21-D7Mit57/Atpla3-D7Mit72/Atp4a. A human Chr 19-enriched cosmid library was screened with both H,K-ATPase α and Na,K-ATPase α3 subunit cDNA probes to isolate the corresponding human genes (ATP4A and ATP1A3, respectively). Fluorescence in situ hybridization with gene-specific cosmid clones localized ATP4A to the q13.1 region, and proximal to ATP1A3, which maps to the q13.2 region, of Chr 19. These results indicate that ATP4A and ATP1A3 are linked in both the mouse and human genomes.

Original languageEnglish (US)
Pages (from-to)644-649
Number of pages6
JournalMammalian Genome
Volume4
Issue number11
DOIs
StatePublished - Nov 1 1993

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Chromosomes, Human, Pair 19
Chromosomes, Human, Pair 7
Proton-Translocating ATPases
Human Chromosomes
Cosmids
Fluorescence In Situ Hybridization
Genes
Inbred Strains Mice
Gene Order
Centromere
Human Genome
Restriction Fragment Length Polymorphisms
Microsatellite Repeats
Complementary DNA
Clone Cells
sodium-translocating ATPase

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Malo, Danielle ; Gros, Philippe ; Bergmann, Anne ; Trask, Barbara ; Mohrenweiser, Harvey W. ; Canfield, Victor A. ; Levenson, Robert. / Genes encoding the H,K-ATPase α and Na,K-ATPase α3 subunits are linked on mouse Chromosome 7 and human Chromosome 19. In: Mammalian Genome. 1993 ; Vol. 4, No. 11. pp. 644-649.
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abstract = "We have used linkage analysis and fluorescence in situ hybridization to determine the chromosomal organization and location of the mouse (Atp4a) and human (ATP4A) genes encoding the H,K-ATPase α subunit. Linkage analysis in recombinant inbred (BXD) strains of mice localized Atp4a to mouse Chromosome (Chr) 7. Segregation of restriction fragment length polymorphisms in backcross progeny of Mus musculusxMus spretus mating confirmed this assignment and indicates that Atp4a and Atp1a3 (gene encoding the murine Na,K-ATPase α3 subunit) are linked and separated by a distance of ∼2 cM. Analysis of the segregation of simple sequence repeats suggested the gene order centromere-D7Mit21-D7Mit57/Atpla3-D7Mit72/Atp4a. A human Chr 19-enriched cosmid library was screened with both H,K-ATPase α and Na,K-ATPase α3 subunit cDNA probes to isolate the corresponding human genes (ATP4A and ATP1A3, respectively). Fluorescence in situ hybridization with gene-specific cosmid clones localized ATP4A to the q13.1 region, and proximal to ATP1A3, which maps to the q13.2 region, of Chr 19. These results indicate that ATP4A and ATP1A3 are linked in both the mouse and human genomes.",
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Genes encoding the H,K-ATPase α and Na,K-ATPase α3 subunits are linked on mouse Chromosome 7 and human Chromosome 19. / Malo, Danielle; Gros, Philippe; Bergmann, Anne; Trask, Barbara; Mohrenweiser, Harvey W.; Canfield, Victor A.; Levenson, Robert.

In: Mammalian Genome, Vol. 4, No. 11, 01.11.1993, p. 644-649.

Research output: Contribution to journalArticle

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T1 - Genes encoding the H,K-ATPase α and Na,K-ATPase α3 subunits are linked on mouse Chromosome 7 and human Chromosome 19

AU - Malo, Danielle

AU - Gros, Philippe

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AU - Trask, Barbara

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AU - Canfield, Victor A.

AU - Levenson, Robert

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