Genes in S. cerevisiae encoding proteins with domains homologous to the mammalian ras proteins

S. Powers, T. Kataoka, O. Fasano, M. Goldfarb, J. Strathem, James Broach, M. Wigler

Research output: Contribution to journalArticle

223 Citations (Scopus)

Abstract

The ras genes, which were first identified by their presence in RNA tumor viruses and which belong to a highly conserved gene family in vertebrates, have two close homologs in yeast, detectable by Southern blotting. We have cloned both genes (RAS1 and RAS2) from plasmid libraries and determined the complete nucleotide sequence of their coding regions. They encode proteins with nearly 90% homology to the first 80 positions of the mammalian ras proteins, and nearly 50% homology to the next 80 amino acids. Yeast RAS1 and RAS2 proteins are more homologous to each other, with about 90% homology for the first 180 positions. After this, at nearly the same position that the mammalian ras proteins begin to diverge from each other, the two yeast ras proteins diverge radically. The yeast ras proteins, like the proteins encoded by the mammalian genes, terminate with the sequence cysAAX, where A is an aliphatic amino acid. Thus the yeast ras proteins have the same overall structure and interrelationship as the family of mammalian ras proteins. The domains of divergence may correspond to functional domains of the ras proteins. Monoclonal antibody directed against mammalian ras proteins immunoprecipitates protein in yeast cells containing high copy numbers of the yeast RAS2 gene.

Original languageEnglish (US)
Pages (from-to)607-612
Number of pages6
JournalCell
Volume36
Issue number3
DOIs
StatePublished - Jan 1 1984

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Saccharomyces cerevisiae Proteins
ras Proteins
Genes
Fungal Proteins
Yeast
Proteins
Yeasts
Amino Acids
ras Genes
Protein Domains
Retroviridae
Southern Blotting
Viruses
Vertebrates
Tumors
Plasmids
Fatty Acids
Nucleotides
Monoclonal Antibodies
Cells

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Powers, S., Kataoka, T., Fasano, O., Goldfarb, M., Strathem, J., Broach, J., & Wigler, M. (1984). Genes in S. cerevisiae encoding proteins with domains homologous to the mammalian ras proteins. Cell, 36(3), 607-612. https://doi.org/10.1016/0092-8674(84)90340-4
Powers, S. ; Kataoka, T. ; Fasano, O. ; Goldfarb, M. ; Strathem, J. ; Broach, James ; Wigler, M. / Genes in S. cerevisiae encoding proteins with domains homologous to the mammalian ras proteins. In: Cell. 1984 ; Vol. 36, No. 3. pp. 607-612.
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Powers, S, Kataoka, T, Fasano, O, Goldfarb, M, Strathem, J, Broach, J & Wigler, M 1984, 'Genes in S. cerevisiae encoding proteins with domains homologous to the mammalian ras proteins', Cell, vol. 36, no. 3, pp. 607-612. https://doi.org/10.1016/0092-8674(84)90340-4

Genes in S. cerevisiae encoding proteins with domains homologous to the mammalian ras proteins. / Powers, S.; Kataoka, T.; Fasano, O.; Goldfarb, M.; Strathem, J.; Broach, James; Wigler, M.

In: Cell, Vol. 36, No. 3, 01.01.1984, p. 607-612.

Research output: Contribution to journalArticle

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