Genes responding to water deficit in apple (Malus × domestica Borkh.) roots

Carole L. Bassett, Angela M. Baldo, Jacob T. Moore, Ryan M. Jenkins, Doug S. Soffe, Michael E. Wisniewski, John L. Norelli, Robert E. Farrell

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Background: Individual plants adapt to their immediate environment using a combination of biochemical, morphological and life cycle strategies. Because woody plants are long-lived perennials, they cannot rely on annual life cycle strategies alone to survive abiotic stresses. In this study we used suppression subtractive hybridization to identify genes both up- and down-regulated in roots during water deficit treatment and recovery. In addition we followed the expression of select genes in the roots, leaves, bark and xylem of 'Royal Gala' apple subjected to a simulated drought and subsequent recovery.Results: In agreement with studies from both herbaceous and woody plants, a number of common drought-responsive genes were identified, as well as a few not previously reported. Three genes were selected for more in depth analysis: a high affinity nitrate transporter (MdNRT2.4), a mitochondrial outer membrane translocase (MdTOM7.1), and a gene encoding an NPR1 homolog (MpNPR1-2). Quantitative expression of these genes in apple roots, bark and leaves was consistent with their roles in nutrition and defense.Conclusions: Additional genes from apple roots responding to drought were identified using suppression subtraction hybridization compared to a previous EST analysis from the same organ. Genes up- and down-regulated during drought recovery in roots were also identified. Elevated levels of a high affinity nitrate transporter were found in roots suggesting that nitrogen uptake shifted from low affinity transport due to the predicted reduction in nitrate concentration in drought-treated roots. Suppression of a NPR1 gene in leaves of drought-treated apple trees may explain in part the increased disease susceptibility of trees subjected to dehydrative conditions.

Original languageEnglish (US)
Article number182
JournalBMC plant biology
Volume14
Issue number1
DOIs
StatePublished - Jul 8 2014

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Malus domestica
apples
drought
genes
water
woody plants
transporters
life cycle (organisms)
bark
nitrates
leaves
gene expression
suppression subtractive hybridization
nitrate reduction
herbaceous plants
abiotic stress
xylem
disease resistance
hybridization
nutrition

All Science Journal Classification (ASJC) codes

  • Plant Science

Cite this

Bassett, C. L., Baldo, A. M., Moore, J. T., Jenkins, R. M., Soffe, D. S., Wisniewski, M. E., ... Farrell, R. E. (2014). Genes responding to water deficit in apple (Malus × domestica Borkh.) roots. BMC plant biology, 14(1), [182]. https://doi.org/10.1186/1471-2229-14-182
Bassett, Carole L. ; Baldo, Angela M. ; Moore, Jacob T. ; Jenkins, Ryan M. ; Soffe, Doug S. ; Wisniewski, Michael E. ; Norelli, John L. ; Farrell, Robert E. / Genes responding to water deficit in apple (Malus × domestica Borkh.) roots. In: BMC plant biology. 2014 ; Vol. 14, No. 1.
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abstract = "Background: Individual plants adapt to their immediate environment using a combination of biochemical, morphological and life cycle strategies. Because woody plants are long-lived perennials, they cannot rely on annual life cycle strategies alone to survive abiotic stresses. In this study we used suppression subtractive hybridization to identify genes both up- and down-regulated in roots during water deficit treatment and recovery. In addition we followed the expression of select genes in the roots, leaves, bark and xylem of 'Royal Gala' apple subjected to a simulated drought and subsequent recovery.Results: In agreement with studies from both herbaceous and woody plants, a number of common drought-responsive genes were identified, as well as a few not previously reported. Three genes were selected for more in depth analysis: a high affinity nitrate transporter (MdNRT2.4), a mitochondrial outer membrane translocase (MdTOM7.1), and a gene encoding an NPR1 homolog (MpNPR1-2). Quantitative expression of these genes in apple roots, bark and leaves was consistent with their roles in nutrition and defense.Conclusions: Additional genes from apple roots responding to drought were identified using suppression subtraction hybridization compared to a previous EST analysis from the same organ. Genes up- and down-regulated during drought recovery in roots were also identified. Elevated levels of a high affinity nitrate transporter were found in roots suggesting that nitrogen uptake shifted from low affinity transport due to the predicted reduction in nitrate concentration in drought-treated roots. Suppression of a NPR1 gene in leaves of drought-treated apple trees may explain in part the increased disease susceptibility of trees subjected to dehydrative conditions.",
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Bassett, CL, Baldo, AM, Moore, JT, Jenkins, RM, Soffe, DS, Wisniewski, ME, Norelli, JL & Farrell, RE 2014, 'Genes responding to water deficit in apple (Malus × domestica Borkh.) roots', BMC plant biology, vol. 14, no. 1, 182. https://doi.org/10.1186/1471-2229-14-182

Genes responding to water deficit in apple (Malus × domestica Borkh.) roots. / Bassett, Carole L.; Baldo, Angela M.; Moore, Jacob T.; Jenkins, Ryan M.; Soffe, Doug S.; Wisniewski, Michael E.; Norelli, John L.; Farrell, Robert E.

In: BMC plant biology, Vol. 14, No. 1, 182, 08.07.2014.

Research output: Contribution to journalArticle

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AU - Farrell, Robert E.

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N2 - Background: Individual plants adapt to their immediate environment using a combination of biochemical, morphological and life cycle strategies. Because woody plants are long-lived perennials, they cannot rely on annual life cycle strategies alone to survive abiotic stresses. In this study we used suppression subtractive hybridization to identify genes both up- and down-regulated in roots during water deficit treatment and recovery. In addition we followed the expression of select genes in the roots, leaves, bark and xylem of 'Royal Gala' apple subjected to a simulated drought and subsequent recovery.Results: In agreement with studies from both herbaceous and woody plants, a number of common drought-responsive genes were identified, as well as a few not previously reported. Three genes were selected for more in depth analysis: a high affinity nitrate transporter (MdNRT2.4), a mitochondrial outer membrane translocase (MdTOM7.1), and a gene encoding an NPR1 homolog (MpNPR1-2). Quantitative expression of these genes in apple roots, bark and leaves was consistent with their roles in nutrition and defense.Conclusions: Additional genes from apple roots responding to drought were identified using suppression subtraction hybridization compared to a previous EST analysis from the same organ. Genes up- and down-regulated during drought recovery in roots were also identified. Elevated levels of a high affinity nitrate transporter were found in roots suggesting that nitrogen uptake shifted from low affinity transport due to the predicted reduction in nitrate concentration in drought-treated roots. Suppression of a NPR1 gene in leaves of drought-treated apple trees may explain in part the increased disease susceptibility of trees subjected to dehydrative conditions.

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Bassett CL, Baldo AM, Moore JT, Jenkins RM, Soffe DS, Wisniewski ME et al. Genes responding to water deficit in apple (Malus × domestica Borkh.) roots. BMC plant biology. 2014 Jul 8;14(1). 182. https://doi.org/10.1186/1471-2229-14-182