Genetic ablation of parietal cells in transgenic mice: A new model for analyzing cell lineage relationships in the gastric mucosa

Victor Canfield, A. Brian West, James R. Goldenring, Robert Levenson

Research output: Contribution to journalArticlepeer-review

97 Scopus citations

Abstract

The gastric mucosa of mammalian stomach contains several differentiated cell types specialized for the secretion of acid, digestive enzymes, mucus, and hormones. Understanding whether each of these cell lineages is derived from a common stem cell has been a challenging problem. We have used a genetic approach to analyze the ontogeny of progenitor cells within mouse stomach. Herpes simplex virus 1 thymidine kinase was targeted to parietal cells within the gastric mucosa of transgenic mice, and parietal cells were ablated by treatment of animals with the antiherpetic drug ganciclovir. Ganciclovir treatment produced complete ablation of parietal cells, dissolution of gastric glands, and loss of chief and mucus-producing cells. Termination of drug treatment led to the reemergence of all major gastric epithelial cell types and restoration of glandular architecture. Our results imply the existence of a pluripotent stem cell for the gastric mucosa. Parietal cell ablation should provide a model for analyzing cell lineage relationships within the stomach as well as mechanisms underlying gastric injury and repair.

Original languageEnglish (US)
Pages (from-to)2431-2435
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume93
Issue number6
DOIs
StatePublished - Mar 19 1996

All Science Journal Classification (ASJC) codes

  • General

Fingerprint Dive into the research topics of 'Genetic ablation of parietal cells in transgenic mice: A new model for analyzing cell lineage relationships in the gastric mucosa'. Together they form a unique fingerprint.

Cite this