Genetic analysis of CRPV pathogenesis: The L1 open reading frame is dispensable for cellular transformation but is required for papilloma formation

M. Nasseri, C. Meyers, F. O. Wettstein

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

Genetic studies to elucidate the role of papillomaviruses in the development and progression of tumors have been severely hampered because the viruses cannot be grown in tissue culture and therefore mutants are not available. We have employed recombinant DNA for papilloma induction to identify essential sequences involved in papillomavirus pathogenesis. Here, we demonstrated that deleting most of the open reading frame (ORF)L2 did not affect the potential of viral cottontail rabbit papillomavirus (CRPV) DNA to induce papillomas. The extrachromosomally maintained DNA in the papillomas was not rearranged and the major transcripts of 1.3 and 2.0 kb encoded E7 and E6, respectively. A recombinant DNA containing a larger deletion lacking the 3′ terminal half of ORF L2 and all of ORF L1 (pdlBcll) did not induce papillomas. The results indicate that sequences in the late region not required for transformation of NIH 3T3 cells by bovine papillomavirus type-1 essential in CRPV for induction of papillomas.

Original languageEnglish (US)
Pages (from-to)321-325
Number of pages5
JournalVirology
Volume170
Issue number1
DOIs
Publication statusPublished - May 1989

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Virology

Cite this