Genetic analysis of helper virus-specific selective amplification of cucumber mosaic virus satellite RNAs

Marilyn J. Roossinck, Peter Palukaitis

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Satellite RNAs (sat-RNAs) are small molecular parasites associated with a number of plant RNA viruses. The cucumber mosaic virus (CMV) sat-RNAs are ca. 335 nucleotides and have evolved to produce a large number of closely related sat-RNAs. Different cucumoviruses can act as helper viruses in the amplification of CMV sat-RNAs. We have found that different helper viruses show a preference for a particular sat-RNA in a mixed infection. In this study the specificity of WL47 sat-RNA amplification by LS-CMV and of D4 sat-RNA amplification by tomato aspermy virus in mixed infections was examined. Recombinant cDNA clones of D4 sat-RNA and WL47 sat-RNA were used to map the sat-RNA sequences responsible for the helper virus selection of a specific sat-RNA for amplification.

Original languageEnglish (US)
Pages (from-to)25-29
Number of pages5
JournalJournal Of Molecular Evolution
Volume40
Issue number1
DOIs
StatePublished - Jan 1 1995

Fingerprint

Cucumber Mosaic Virus Satellite
satellite RNA
Helper Viruses
Cucumber mosaic virus
genetic analysis
Viruses
Amplification
genetic techniques and protocols
RNA
amplification
virus
viruses
Satellite RNAs
Cucumovirus
Coinfection
mixed infection
Tomato aspermy virus
Plant RNA
Plant Viruses
RNA Viruses

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics

Cite this

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Genetic analysis of helper virus-specific selective amplification of cucumber mosaic virus satellite RNAs. / Roossinck, Marilyn J.; Palukaitis, Peter.

In: Journal Of Molecular Evolution, Vol. 40, No. 1, 01.01.1995, p. 25-29.

Research output: Contribution to journalArticle

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