Genetic analysis of the RNA polymerase II CTD in Drosophila

Feiyue Lu, David S. Gilmour

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Abstract

The Carboxy-terminal Domain (CTD) of RNA polymerase II (Pol II) plays essential roles in regulating gene expression in eukaryotes. Here, we describe multiple genetic approaches for studying the CTD in Drosophila that complement pre-existing molecular analyses of the Pol II CTD in other experimental models. These approaches will allow one to assess the effects of any CTD mutations in a developmentally complex organism. The approaches discussed in this work can in principle, be applied to analyze other transcription components in eukaryotes.

Original languageEnglish (US)
Pages (from-to)129-137
Number of pages9
JournalMethods
Volume159-160
DOIs
StatePublished - Apr 15 2019

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

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