Genetic analysis of yeast RAS1 and RAS2 genes

T. Kataoka, S. Powers, C. McGill, O. Fasano, J. Strathern, James Broach, M. Wigler

Research output: Contribution to journalArticle

259 Citations (Scopus)

Abstract

We present a genetic analysis of RAS1 and RAS2 of S. cerevisiae, two genes that are highly homologous to mammalian ras genes. By constructing in vitro ras genes disrupted by selectable genes and introducing these by gene replacement into the respective ras loci, we have determined that neither RAS1 nor RAS2 are by themselves essential genes. However, rasl- ras2- spores of doubly heterozygous diploids are incapable of resuming vegetative growth. We have determined that RAS1 is located on chromosome XV, 7 cM from ade2 and 63 cM from his3; and RAS2 is located on chromosome XIV, 2 cM from met4. We have also constructed by site-directed mutagenesis a missense mutant, RAS2val19, which encodes valine in place of glycine at the nineteenth amino acid position, the same sort of missense mutation that is found in some transforming alleles of mammalian ras genes. Diploid yeast cells that contain this mutation are incapable of sporulating efficiently, even when they contain wild-type alleles.

Original languageEnglish (US)
Pages (from-to)437-445
Number of pages9
JournalCell
Volume37
Issue number2
DOIs
StatePublished - Jan 1 1984

Fingerprint

ras Genes
Yeast
Genes
Yeasts
Diploidy
Alleles
Chromosomes, Human, Pair 7
Chromosomes, Human, Pair 2
Essential Genes
Valine
Missense Mutation
Site-Directed Mutagenesis
Spores
Glycine
Chromosomes
Saccharomyces cerevisiae
Amino Acids
Mutation
Mutagenesis
Growth

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Kataoka, T., Powers, S., McGill, C., Fasano, O., Strathern, J., Broach, J., & Wigler, M. (1984). Genetic analysis of yeast RAS1 and RAS2 genes. Cell, 37(2), 437-445. https://doi.org/10.1016/0092-8674(84)90374-X
Kataoka, T. ; Powers, S. ; McGill, C. ; Fasano, O. ; Strathern, J. ; Broach, James ; Wigler, M. / Genetic analysis of yeast RAS1 and RAS2 genes. In: Cell. 1984 ; Vol. 37, No. 2. pp. 437-445.
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Kataoka, T, Powers, S, McGill, C, Fasano, O, Strathern, J, Broach, J & Wigler, M 1984, 'Genetic analysis of yeast RAS1 and RAS2 genes', Cell, vol. 37, no. 2, pp. 437-445. https://doi.org/10.1016/0092-8674(84)90374-X

Genetic analysis of yeast RAS1 and RAS2 genes. / Kataoka, T.; Powers, S.; McGill, C.; Fasano, O.; Strathern, J.; Broach, James; Wigler, M.

In: Cell, Vol. 37, No. 2, 01.01.1984, p. 437-445.

Research output: Contribution to journalArticle

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N2 - We present a genetic analysis of RAS1 and RAS2 of S. cerevisiae, two genes that are highly homologous to mammalian ras genes. By constructing in vitro ras genes disrupted by selectable genes and introducing these by gene replacement into the respective ras loci, we have determined that neither RAS1 nor RAS2 are by themselves essential genes. However, rasl- ras2- spores of doubly heterozygous diploids are incapable of resuming vegetative growth. We have determined that RAS1 is located on chromosome XV, 7 cM from ade2 and 63 cM from his3; and RAS2 is located on chromosome XIV, 2 cM from met4. We have also constructed by site-directed mutagenesis a missense mutant, RAS2val19, which encodes valine in place of glycine at the nineteenth amino acid position, the same sort of missense mutation that is found in some transforming alleles of mammalian ras genes. Diploid yeast cells that contain this mutation are incapable of sporulating efficiently, even when they contain wild-type alleles.

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Kataoka T, Powers S, McGill C, Fasano O, Strathern J, Broach J et al. Genetic analysis of yeast RAS1 and RAS2 genes. Cell. 1984 Jan 1;37(2):437-445. https://doi.org/10.1016/0092-8674(84)90374-X